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Synthesis, Characterization, and Antibacterial Activity of Cross-Linked Chitosan-Glutaraldehyde
State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China
State Key Laboratory Breeding Base for Zhejiang Sustainable Plant Pest and Disease Control, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua 321004, China
Institute of Digital Agriculture, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
Research and Development Center of Biorational Pesticides, Northwest A & F University, Yangling, Shaanxi 712100, China
State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China
* Authors to whom correspondence should be addressed.
Received: 18 February 2013; in revised form: 17 April 2013 / Accepted: 26 April 2013 / Published: 13 May 2013
Abstract: This present study deals with synthesis, characterization and antibacterial activity of cross-linked chitosan-glutaraldehyde. Results from this study indicated that cross-linked chitosan-glutaraldehyde markedly inhibited the growth of antibiotic-resistant Burkholderia cepacia complex regardless of bacterial species and incubation time while bacterial growth was unaffected by solid chitosan. Furthermore, high temperature treated cross-linked chitosan-glutaraldehyde showed strong antibacterial activity against the selected strain 0901 although the inhibitory effects varied with different temperatures. In addition, physical-chemical and structural characterization revealed that the cross-linking of chitosan with glutaraldehyde resulted in a rougher surface morphology, a characteristic Fourier transform infrared (FTIR) band at 1559 cm−1, a specific X-ray diffraction peak centered at 2θ = 15°, a lower contents of carbon, hydrogen and nitrogen, and a higher stability of glucose units compared to chitosan based on scanning electron microscopic observation, FTIR spectra, X-ray diffraction pattern, as well as elemental and thermo gravimetric analysis. Overall, this study indicated that cross-linked chitosan-glutaraldehyde is promising to be developed as a new antibacterial drug.
Keywords: antibacterial activity; characterization; chitosan; cross-link; glutaraldehyde
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MDPI and ACS Style
Li, B.; Shan, C.-L.; Zhou, Q.; Fang, Y.; Wang, Y.-L.; Xu, F.; Han, L.-R.; Ibrahim, M.; Guo, L.-B.; Xie, G.-L.; Sun, G.-C. Synthesis, Characterization, and Antibacterial Activity of Cross-Linked Chitosan-Glutaraldehyde. Mar. Drugs 2013, 11, 1534-1552.
Li B, Shan C-L, Zhou Q, Fang Y, Wang Y-L, Xu F, Han L-R, Ibrahim M, Guo L-B, Xie G-L, Sun G-C. Synthesis, Characterization, and Antibacterial Activity of Cross-Linked Chitosan-Glutaraldehyde. Marine Drugs. 2013; 11(5):1534-1552.
Li, Bin; Shan, Chang-Lin; Zhou, Qing; Fang, Yuan; Wang, Yang-Li; Xu, Fei; Han, Li-Rong; Ibrahim, Muhammad; Guo, Long-Biao; Xie, Guan-Lin; Sun, Guo-Chang. 2013. "Synthesis, Characterization, and Antibacterial Activity of Cross-Linked Chitosan-Glutaraldehyde." Mar. Drugs 11, no. 5: 1534-1552.