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Chemoecological Screening Reveals High Bioactivity in Diverse Culturable Portuguese Marine Cyanobacteria
CIMAR/CIIMAR—Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Rua dos Bragas 177, Porto 4050-123, Portugal
Department of Biology, Faculty of Sciences, University of Porto, Rua do Campo Alegre, Porto 4169-007, Portugal
Scripps Institution of Oceanography, University of California, San Diego, La Jolla, CA 92093, USA
Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California, San Diego, La Jolla, CA 92093, USA
* Author to whom correspondence should be addressed.
Received: 7 January 2013; in revised form: 12 March 2013 / Accepted: 27 March 2013 / Published: 22 April 2013
Abstract: Marine cyanobacteria, notably those from tropical regions, are a rich source of bioactive secondary metabolites. Tropical marine cyanobacteria often grow to high densities in the environment, allowing direct isolation of many secondary metabolites from field-collected material. However, in temperate environments culturing is usually required to produce enough biomass for investigations of their chemical constituents. In this work, we cultured a selection of novel and diverse cyanobacteria isolated from the Portuguese coast, and tested their organic extracts in a series of ecologically-relevant bioassays. The majority of the extracts showed activity in at least one of the bioassays, all of which were run in very small scale. Phylogenetically related isolates exhibited different activity profiles, highlighting the value of microdiversity for bioprospection studies. Furthermore, LC-MS analyses of selected active extracts suggested the presence of previously unidentified secondary metabolites. Overall, the screening strategy employed here, in which previously untapped cyanobacterial diversity was combined with multiple bioassays, proved to be a successful strategy and allowed the selection of several strains for further investigations based on their bioactivity profiles.
Keywords: cyanobacteria; chemical ecology; screening; bioactive compounds; secondary metabolites; phylogenetic analysis
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MDPI and ACS Style
Leão, P.N.; Ramos, V.; Gonçalves, P.B.; Viana, F.; Lage, O.M.; Gerwick, W.H.; Vasconcelos, V.M. Chemoecological Screening Reveals High Bioactivity in Diverse Culturable Portuguese Marine Cyanobacteria. Mar. Drugs 2013, 11, 1316-1335.
Leão PN, Ramos V, Gonçalves PB, Viana F, Lage OM, Gerwick WH, Vasconcelos VM. Chemoecological Screening Reveals High Bioactivity in Diverse Culturable Portuguese Marine Cyanobacteria. Marine Drugs. 2013; 11(4):1316-1335.
Leão, Pedro N.; Ramos, Vitor; Gonçalves, Patrício B.; Viana, Flávia; Lage, Olga M.; Gerwick, William H.; Vasconcelos, Vitor M. 2013. "Chemoecological Screening Reveals High Bioactivity in Diverse Culturable Portuguese Marine Cyanobacteria." Mar. Drugs 11, no. 4: 1316-1335.