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A Therapeutic Potential for Marine Skeletal Proteins in Bone Regeneration
Department of Chemistry and Forensic Science, University of Technology Sydney, Ultimo, NSW 2007, Australia
Proteomics Core Facility, Faculty of Science, University of Technology Sydney, Ultimo, NSW 2007, Australia
Current address: Department of Oral Biosciences, Faculty of Dentistry, University of Hong Kong, The Prince Philip Dental Hospital, 34 Hospital Road, Sai Ying Pun, Hong Kong.
* Author to whom correspondence should be addressed.
Received: 18 January 2013; in revised form: 13 March 2013 / Accepted: 1 April 2013 / Published: 10 April 2013
Abstract: A vital ingredient for engineering bone tissue, in the culture dish, is the use of recombinant matrix and growth proteins to help accelerate the growth of cultivated tissues into clinically acceptable quantities. The skeletal organic matrices of calcifying marine invertebrates are an untouched potential source of such growth inducing proteins. They have the advantage of being ready-made and retain the native state of the original protein. Striking evidence shows that skeleton building bone morphogenic protein-2/4 (BMP) and transforming growth factor beta (TGF-β) exist within various marine invertebrates such as, corals. Best practice mariculture and the latest innovations in long-term marine invertebrate cell cultivation can be implemented to ensure that these proteins are produced sustainably and supplied continuously. This also guarantees that coral reef habitats are not damaged during the collection of specimens. Potential proteins for bone repair, either extracted from the skeleton or derived from cultivated tissues, can be identified, evaluated and retrieved using chromatography, cell assays and proteomic methods. Due to the current evidence for bone matrix protein analogues in marine invertebrates, together with the methods established for their production and retrieval there is a genuine prospect that they can be used to regenerate living bone for potential clinical use.
Keywords: proteomics; bone tissue engineering; mesenchymal stem cells; marine invertebrate skeletons; bone matrix proteins
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MDPI and ACS Style
Green, D.W.; Padula, M.P.; Santos, J.; Chou, J.; Milthorpe, B.; Ben-Nissan, B. A Therapeutic Potential for Marine Skeletal Proteins in Bone Regeneration. Mar. Drugs 2013, 11, 1203-1220.
Green DW, Padula MP, Santos J, Chou J, Milthorpe B, Ben-Nissan B. A Therapeutic Potential for Marine Skeletal Proteins in Bone Regeneration. Marine Drugs. 2013; 11(4):1203-1220.
Green, David W.; Padula, Matthew P.; Santos, Jerran; Chou, Joshua; Milthorpe, Bruce; Ben-Nissan, Besim. 2013. "A Therapeutic Potential for Marine Skeletal Proteins in Bone Regeneration." Mar. Drugs 11, no. 4: 1203-1220.