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Mar. Drugs 2013, 11(12), 4751-4760; doi:10.3390/md11124751
Article

Okadaic Acid Toxin at Sublethal Dose Produced Cell Proliferation in Gastric and Colon Epithelial Cell Lines

1
, 2
 and 1,*
Received: 31 August 2013; in revised form: 29 October 2013 / Accepted: 12 November 2013 / Published: 2 December 2013
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Abstract: The aim of this study was to analyze the effect of Okadaic Acid (OA) on the proliferation of gastric and colon epithelial cells, the main target tissues of the toxin. We hypothesized that OA, at sublethal doses, activates multiple signaling pathways, such as Erk and Akt, through the inhibition of PP2A. To demonstrate this, we carried out curves of doses and time response against OA in AGS, MKN-45 and Caco 2 cell lines, and found an increase in the cell proliferation at sublethal doses, at 24 h or 48 h exposure. Indeed, cells can withstand high concentrations of the toxin at 4 h exposure, the time chosen considering the maximum time before total gastric emptying. We have proved that this increased proliferation is due to an overexpression of Cyclin B, a cyclin that promotes the passage from G2 to mitosis. In addition, we have demonstrated that OA induces activation of Akt and Erk in the three cells lines, showing that OA can activate pathways involved in oncogenesis. In conclusion, this study contributes to the knowledge about the possible effects of chronic OA consumption.
Keywords: okadaic acid; gastric cancer; colon cancer; PP2A; proliferation okadaic acid; gastric cancer; colon cancer; PP2A; proliferation
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

del Campo, M.; Toledo, H.; Lagos, N. Okadaic Acid Toxin at Sublethal Dose Produced Cell Proliferation in Gastric and Colon Epithelial Cell Lines. Mar. Drugs 2013, 11, 4751-4760.

AMA Style

del Campo M, Toledo H, Lagos N. Okadaic Acid Toxin at Sublethal Dose Produced Cell Proliferation in Gastric and Colon Epithelial Cell Lines. Marine Drugs. 2013; 11(12):4751-4760.

Chicago/Turabian Style

del Campo, Miguel; Toledo, Héctor; Lagos, Néstor. 2013. "Okadaic Acid Toxin at Sublethal Dose Produced Cell Proliferation in Gastric and Colon Epithelial Cell Lines." Mar. Drugs 11, no. 12: 4751-4760.


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