Mar. Drugs 2012, 10(8), 1852-1872; doi:10.3390/md10081852
Article

Pardaxin, a Fish Antimicrobial Peptide, Exhibits Antitumor Activity toward Murine Fibrosarcoma in Vitro and in Vivo

Shu-Ping Wu 1,† email, Tsui-Chin Huang 2,† email, Ching-Chun Lin 3 email, Cho-Fat Hui 3,* email, Cheng-Hui Lin 1 email and Jyh-Yih Chen 2,* email
1 Department of Aquaculture, National Taiwan Ocean University, Keelung 202, Taiwan 2 Marine Research Station, Institute of Cellular and Organismic Biology, Academia Sinica, 23-10 Dahuen Rd., Jiaushi, Ilan 262, Taiwan 3 Institute of Cellular and Organismic Biology, Academia Sinica, Taipei 115, Taiwan † These authors contributed equally to this work.
* Authors to whom correspondence should be addressed.
Received: 19 June 2012; in revised form: 18 July 2012 / Accepted: 14 August 2012 / Published: 22 August 2012
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Abstract: The antitumor activity of pardaxin, a fish antimicrobial peptide, has not been previously examined in in vitro and in vivo systems for treating murine fibrosarcoma. In this study, the antitumor activity of synthetic pardaxin was tested using murine MN-11 tumor cells as the study model. We show that pardaxin inhibits the proliferation of MN-11 cells and reduces colony formation in a soft agar assay. Transmission electron microscopy (TEM) showed that pardaxin altered the membrane structure similar to what a lytic peptide does, and also produced apoptotic features, such as hollow mitochondria, nuclear condensation, and disrupted cell membranes. A qRT-PCR and ELISA showed that pardaxin induced apoptosis, activated caspase-7 and interleukin (IL)-7r, and downregulated caspase-9, ATF 3, SOCS3, STAT3, cathelicidin, p65, and interferon (IFN)-γ suggesting that pardaxin induces apoptosis through the death receptor/nuclear factor (NF)-κB signaling pathway after 14 days of treatment in tumor-bearing mice. An antitumor effect was observed when pardaxin (25 mg/kg; 0.5 mg/day) was used to treat mice for 14 days, which caused significant inhibition of MN-11 cell growth in mice. Overall, these results indicate that pardaxin has the potential to be a novel therapeutic agent to treat fibrosarcomas.
Keywords: pardaxin; fibrosarcoma; antitumor

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MDPI and ACS Style

Wu, S.-P.; Huang, T.-C.; Lin, C.-C.; Hui, C.-F.; Lin, C.-H.; Chen, J.-Y. Pardaxin, a Fish Antimicrobial Peptide, Exhibits Antitumor Activity toward Murine Fibrosarcoma in Vitro and in Vivo. Mar. Drugs 2012, 10, 1852-1872.

AMA Style

Wu S-P, Huang T-C, Lin C-C, Hui C-F, Lin C-H, Chen J-Y. Pardaxin, a Fish Antimicrobial Peptide, Exhibits Antitumor Activity toward Murine Fibrosarcoma in Vitro and in Vivo. Marine Drugs. 2012; 10(8):1852-1872.

Chicago/Turabian Style

Wu, Shu-Ping; Huang, Tsui-Chin; Lin, Ching-Chun; Hui, Cho-Fat; Lin, Cheng-Hui; Chen, Jyh-Yih. 2012. "Pardaxin, a Fish Antimicrobial Peptide, Exhibits Antitumor Activity toward Murine Fibrosarcoma in Vitro and in Vivo." Mar. Drugs 10, no. 8: 1852-1872.

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