Mar. Drugs 2012, 10(6), 1307-1320; doi:10.3390/md10061307
Article

Marine Cyclotripeptide X-13 Promotes Angiogenesis in Zebrafish and Human Endothelial Cells via PI3K/Akt/eNOS Signaling Pathways

Xi-Lin Lu 1,† email, Zhong-Liang Xu 2,† email, Xiao-Li Yao 1 email, Feng-Juan Su 1 email, Cheng-Hui Ye 1 email, Jing Li 2 email, Yong-Cheng Lin 2 email, Guang-Lei Wang 3,4 email, Jin-Sheng Zeng 1 email, Ru-Xun Huang 1 email, Jing-Song Ou 5,6 email, Hong-Shuo Sun 7 email, Li-Ping Wang 8 email, Ji-Yan Pang 2,* email and Zhong Pei 1,* email
1 Department of Neurology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China 2 School of Chemistry & Chemical Engineering, Sun Yat-sen University, Guangzhou 510275, China 3 Department of Pharmacology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, China 4 Key Laboratory of Functional Molecules from Oceanic Microorganisms, Department of Education of Guangdong Province, Guangzhou 510080, China 5 Division of Cardiac Surgery, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China 6 The Key Laboratory of Assisted Circulation, Ministry of Health, Guangzhou 510182, China 7 Division of Anatomy, Department of Surgery, Institute of Medical Science, Faculty of Medicine, University of Toronto, Toronto, ON M5G 1G6, Canada 8 Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China † These authors contributed equally to this work.
* Authors to whom correspondence should be addressed.
Received: 4 March 2012; in revised form: 8 May 2012 / Accepted: 29 May 2012 / Published: 7 June 2012
PDF Full-text Download PDF Full-Text [1572 KB, uploaded 7 June 2012 14:38 CEST]
Abstract: Cyclotripeptide X-13 is a core of novel marine compound xyloallenoide A isolated from mangrove fungus Xylaria sp. (no. 2508). We found that X-13 dose-dependently induced angiogenesis in zebrafish embryos and in human endothelial cells, which was accompanied by increased phosphorylation of eNOS and Akt and NO release. Inhibition of PI3K/Akt/eNOS by LY294002 or l-NAME suppressed X-13-induced angiogenesis. The present work demonstrates that X-13 promotes angiogenesis via PI3K/Akt/eNOS pathways.
Keywords: angiogenesis; marine cyclotripeptide; zebrafish; endothelial nitric oxide synthase; xyloallenoide A

Article Statistics

Click here to load and display the download statistics.

Cite This Article

MDPI and ACS Style

Lu, X.-L.; Xu, Z.-L.; Yao, X.-L.; Su, F.-J.; Ye, C.-H.; Li, J.; Lin, Y.-C.; Wang, G.-L.; Zeng, J.-S.; Huang, R.-X.; Ou, J.-S.; Sun, H.-S.; Wang, L.-P.; Pang, J.-Y.; Pei, Z. Marine Cyclotripeptide X-13 Promotes Angiogenesis in Zebrafish and Human Endothelial Cells via PI3K/Akt/eNOS Signaling Pathways. Mar. Drugs 2012, 10, 1307-1320.

AMA Style

Lu X-L, Xu Z-L, Yao X-L, Su F-J, Ye C-H, Li J, Lin Y-C, Wang G-L, Zeng J-S, Huang R-X, Ou J-S, Sun H-S, Wang L-P, Pang J-Y, Pei Z. Marine Cyclotripeptide X-13 Promotes Angiogenesis in Zebrafish and Human Endothelial Cells via PI3K/Akt/eNOS Signaling Pathways. Marine Drugs. 2012; 10(6):1307-1320.

Chicago/Turabian Style

Lu, Xi-Lin; Xu, Zhong-Liang; Yao, Xiao-Li; Su, Feng-Juan; Ye, Cheng-Hui; Li, Jing; Lin, Yong-Cheng; Wang, Guang-Lei; Zeng, Jin-Sheng; Huang, Ru-Xun; Ou, Jing-Song; Sun, Hong-Shuo; Wang, Li-Ping; Pang, Ji-Yan; Pei, Zhong. 2012. "Marine Cyclotripeptide X-13 Promotes Angiogenesis in Zebrafish and Human Endothelial Cells via PI3K/Akt/eNOS Signaling Pathways." Mar. Drugs 10, no. 6: 1307-1320.

Mar. Drugs EISSN 1660-3397 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert