Mar. Drugs 2012, 10(5), 1066-1080; doi:10.3390/md10051066
Article

Optimization of Hydrolysis Conditions for the Production of Angiotensin-I Converting Enzyme-Inhibitory Peptides and Isolation of a Novel Peptide from Lizard Fish (Saurida elongata) Muscle Protein Hydrolysate

1 School of Chemistry and Chemical Engineering, Guangxi University, Nanning, Guangxi 530004, China 2 Department of Pharmacy, Liuzhou Medical College, Liuzhou, Guangxi 545006, China 3 Guangxi Key Laboratory of Petrochemical Resources Processing & Process Intensification Technology, Nanning, Guangxi 530004, China
* Author to whom correspondence should be addressed.
Received: 2 March 2012; in revised form: 26 April 2012 / Accepted: 4 May 2012 / Published: 18 May 2012
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Abstract: Lizard fish (Saurida elongata) muscle protein was hydrolyzed using neutral protease to produce protein hydrolysate (LFPH), and the hydrolysis conditions were investigated using response-surface methodology. The optimum conditions for producing peptides with the highest angiotensin-I converting enzyme (ACE)-inhibitory activity were the following: enzyme-to-substrate ratio of 10,000 U/g, temperature of 48 °C, pH 7.0, and hydrolysis time of 2 h. Under these conditions, the ACE-inhibitory activity of LFPH and the degree of hydrolysis were 84% and 24%, respectively. A novel ACE-inhibitory peptide was isolated from LFPH using ultrafiltration, Sephadex G-15, and high-performance liquid chromatography. The amino acid sequence of the ACE-inhibitory peptide was identified as Ser-Pro-Arg-Cys-Arg (SPRCR), and its IC50 was 41 ± 1 µM.
Keywords: ACE-inhibitory peptide; lizard fish; enzymatic hydrolysis; response surface methodology; isolation

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MDPI and ACS Style

Wu, S.; Sun, J.; Tong, Z.; Lan, X.; Zhao, Z.; Liao, D. Optimization of Hydrolysis Conditions for the Production of Angiotensin-I Converting Enzyme-Inhibitory Peptides and Isolation of a Novel Peptide from Lizard Fish (Saurida elongata) Muscle Protein Hydrolysate. Mar. Drugs 2012, 10, 1066-1080.

AMA Style

Wu S, Sun J, Tong Z, Lan X, Zhao Z, Liao D. Optimization of Hydrolysis Conditions for the Production of Angiotensin-I Converting Enzyme-Inhibitory Peptides and Isolation of a Novel Peptide from Lizard Fish (Saurida elongata) Muscle Protein Hydrolysate. Marine Drugs. 2012; 10(5):1066-1080.

Chicago/Turabian Style

Wu, Shanguang; Sun, Jianhua; Tong, Zhangfa; Lan, Xiongdiao; Zhao, Zhongxing; Liao, Dankui. 2012. "Optimization of Hydrolysis Conditions for the Production of Angiotensin-I Converting Enzyme-Inhibitory Peptides and Isolation of a Novel Peptide from Lizard Fish (Saurida elongata) Muscle Protein Hydrolysate." Mar. Drugs 10, no. 5: 1066-1080.

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