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Mar. Drugs 2012, 10(5), 1081-1091; doi:10.3390/md10051081
Article

Pullularins E and F, Two New Peptides from the Endophytic Fungus Bionectria ochroleuca Isolated from the Mangrove Plant Sonneratia caseolaris

1,2,†
, 1,†
, 1
, 3
, 4
, 5
, 1,*  and 1,*
1 Institute of Pharmaceutical Biology and Biotechnology, Heinrich-Heine University, Universitaetsstrasse 1, D-40225 Duesseldorf, Germany 2 Department of Pharmacognosy, Faculty of Pharmacy, Mansoura University, Mansoura 35516, Egypt 3 Helmholtz Centre for Infection Research, Inhoffenstraße 7, Braunschweig D-38124, Germany 4 National Research Laboratories of Natural and Biomimetic Drugs, Health Science Center, Peking University, Beijing 100083, China 5 Marine Biodiscovery Centre, University of Aberdeen, Meston Walk, Aberdeen, Scotland AB24 3UE, UK These authors contributed equally to this work.
* Authors to whom correspondence should be addressed.
Received: 27 March 2012 / Revised: 11 May 2012 / Accepted: 11 May 2012 / Published: 18 May 2012
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Abstract

Chemical investigation of the EtOAc extract of the endophytic fungus Bionectria ochroleuca, isolated from the inner leaf tissues of the plant Sonneratia caseolaris (Sonneratiaceae) from Hainan island (China), yielded two new peptides, pullularins E and F (1 and 2) together with three known compounds (35). The structures of the new compounds were unambiguously determined on the basis of one- and two-dimensional NMR spectroscopy as well as by high-resolution mass spectrometry. The absolute configurations of amino acids were determined by HPLC analysis of acid hydrolysates using Marfey’s method. The isolated compounds exhibited pronounced to moderate cytotoxic activity against the mouse lymphoma cells (L5178Y) with EC50 values ranging between 0.1 and 6.7 µg/mL.
Keywords: mangrove plants; Sonneratia; endophytes; Bionectria; peptide; structure elucidation; Marfey’s method mangrove plants; Sonneratia; endophytes; Bionectria; peptide; structure elucidation; Marfey’s method
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Ebrahim, W.; Kjer, J.; El Amrani, M.; Wray, V.; Lin, W.; Ebel, R.; Lai, D.; Proksch, P. Pullularins E and F, Two New Peptides from the Endophytic Fungus Bionectria ochroleuca Isolated from the Mangrove Plant Sonneratia caseolaris. Mar. Drugs 2012, 10, 1081-1091.

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