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Steroids from the Soft Coral Sinularia crassa
Chinese Medicinal Research and Development Center, China Medical University Hospital, Taichung 40402, Taiwan
China Medical University, Taichung, 40402, Taiwan
Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung 80424, Taiwan
Asian Pacific Ocean Research Center, National Sun Yat-sen University, Kaohsiung 80424, Taiwan
Graduate Institute of Integrated Medicine, College of Chinese Medicine, China Medical University, Taichung 40402, Taiwan
Institute of Oceanography, National Taiwan University, Taipei, 10617, Taiwan
* Author to whom correspondence should be addressed.
Received: 9 January 2012; in revised form: 7 February 2012 / Accepted: 9 February 2012 / Published: 16 February 2012
Abstract: One new sterol, crassarosterol A (1), and four new steroidal glycosides, crassarosterosides A–D (2–5) were isolated from the Formosan soft coral Sinularia crassa. The absolute configuration of 1 was determined using the Mosher’s method. The absolute configurations for the sugar moieties of 2–5 were determined by HPLC analysis on the o-tolylthiocarbamates derived from the liberated sugar after acid hydrolysis. Compounds 2 and 4 could significantly inhibit the expression of pro-inflammatory iNOS protein at 10 µM. In contrast, 1–3 were found to stimulate the expression of COX-2 protein at this concentration. Steroids 1 and 4 also showed cytotoxicity toward the selected human liver cancer cells.
Keywords: Sinularia crassa; crassarosterosides A–D; crassarosterol A; anti-inflammatory activity; o-tolylthiocarbamate
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Chao, C.-H.; Chou, K.-J.; Huang, C.-Y.; Wen, Z.-H.; Hsu, C.-H.; Wu, Y.-C.; Dai, C.-F.; Sheu, J.-H. Steroids from the Soft Coral Sinularia crassa . Mar. Drugs 2012, 10, 439-450.
Chao C-H, Chou K-J, Huang C-Y, Wen Z-H, Hsu C-H, Wu Y-C, Dai C-F, Sheu J-H. Steroids from the Soft Coral Sinularia crassa . Marine Drugs. 2012; 10(2):439-450.
Chao, Chih-Hua; Chou, Kuei-Ju; Huang, Chiung-Yao; Wen, Zhi-Hong; Hsu, Chi-Hsin; Wu, Yang-Chang; Dai, Chang-Feng; Sheu, Jyh-Horng. 2012. "Steroids from the Soft Coral Sinularia crassa ." Mar. Drugs 10, no. 2: 439-450.