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Mar. Drugs 2012, 10(1), 35-50; doi:10.3390/md10010035

Purification, Characterization and Antitumor Activities of a New Protein from Syngnathus acus, an Officinal Marine Fish

1 School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China 2 School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China
* Author to whom correspondence should be addressed.
Received: 2 November 2011 / Revised: 5 December 2011 / Accepted: 14 December 2011 / Published: 30 December 2011
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Discovery and development of new antitumor agents from abundant marine fish are attracting an increasing interest. In the present study, we extracted and purified a novel antitumor protein Syngnathusin from the whole body of Syngnathus acus L., a precious marine fish traditionally used for tumors. Syngnathusin was comprised of 16 kinds of amino acids, mainly acidic amino acids. Its molecular weight was 67.3 kDa and its isoelectric point was 4.57. The N-terminal amino acid sequence of Syngnathusin was determined to be Lys-Arg-Asp-Leu-Gly-Phe-Val-Asp-Glu-Ile-Ser-Ala-His-Tyr and showed no significant homology with the known proteins. Syngnathusin could significantly inhibit the growth of A549 and CCRF-CEM cells. However, the obvious proliferation inhibition against human non-tumor cell lines was not observed. Flow cytometry, morphologic assessment and comet assay revealed that Syngnathusin could induce apoptosis in A549 and CCRF-CEM cells and strongly cooperated with MTX. Syngnathusin could inhibit the growth of S180 tumor transplanted in mice. Syngnathusin may be developed as a novel, selective and effective antineoplastic agent.
Keywords: Syngnathus acus; purification; characterization; antitumor; protein Syngnathus acus; purification; characterization; antitumor; protein
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Wang, M.; Nie, Y.; Peng, Y.; He, F.; Yang, J.; Wu, C.; Li, X. Purification, Characterization and Antitumor Activities of a New Protein from Syngnathus acus, an Officinal Marine Fish. Mar. Drugs 2012, 10, 35-50.

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