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Pharmaceuticals 2013, 6(7), 837-850; doi:10.3390/ph6070837

Diamidines versus Monoamidines as Anti-Pneumocystis Agents: An in Vivo Study
Dimitri Stanicki 1, Muriel Pottier 2, Nausicaa Gantois 2, Claire Pinçon 3, Delphine Forge 1, Isabelle Mahieu 4, Sébastien Boutry 4, Jean Jacques Vanden Eynde 1,*, Anna Martinez 2, Eduardo Dei-Cas 2,5 and El-Moukhtar Aliouat 2
Laboratory of Organic Chemistry, University of Mons-UMONS, B-7000 Mons, Belgium
Biology & Diversity of Emerging Eukaryotic Pathogens (BDEEP), Center for Infection and Immunity of Lille (CIIL), INSERM U1019, CNRS UMR 8204, EA-4547, Univ Lille Nord de France, UDSL, Institut Pasteur de Lille, F-59000 Lille, France
EA2694, Department of Biostatistics, Université de Lille Nord de France, F-59000 Lille, France
Department of General, Organic, and Biomedical Chemistry, NMR and Molecular Imaging Laboratory, University of Mons-UMONS, B-7000 Mons, Belgium
CHU Lille, Biology & Pathology Center, Parasitology-Mycology, F-59000 Lille, France
Author to whom correspondence should be addressed; Tel.: +32-65-373337.
Received: 22 April 2013; in revised form: 14 May 2013 / Accepted: 17 June 2013 / Published: 1 July 2013


: Some compounds articulated around a piperazine or an ethylenediamine linker have been evaluated in vitro to determine their activity in the presence of a 3T6 fibroblast cell line and an axenic culture of Pneumocystis carinii, respectively. The most efficient antifungal derivatives, namely N,N′-bis(benzamidine-4-yl)ethane-1,2-diamine (compound 6, a diamidine) and N-(benzamidine-4-yl)-N′-phenylethane-1,2-diamine (compound 7, a monoamidine), exhibited no cytotoxicity and were evaluated in vivo in a rat model. Only the diamidine 6 emerged as a promising hit for further studies.
Pneumocystis; pentamidine; diamidine

1. Introduction

Pneumocystis fungus lives in the lungs of mammals without developing any risk for healthy individuals. Anarchic proliferation of Pneumocystis however is observed in immunosuppressed populations and can induce a severe interstitial lung disease known as Pneumocystis pneumonia (PcP) or pneumocystosis [1,2]. That opportunistic illness is widely recognized as one of the initial signs of a HIV infection, but it also affects transplant recipients and patients with malignancies, as well as those receiving chronic corticosteroid therapy or suffering from protein malnutrition, connective tissue diseases, acute lymphatic leukaemia, etc. [3]. Pneumocystis could also be responsible for upper respiratory tract problems in very young immunocompetent children [4]. Despite its major impact on public health, no continuous in vitro model allows to grow Pneumocystis for a long period of time, implying that most researches have to rely on animal models of pneumocystosis.

There are few drugs in clinical use that are effective against Pneumocystis [2,5]. The well-known trimethoprim-sulfamethoxazole association constitutes the privileged treatment, but failure occurs in as many as 20 percent of cases. In addition many adverse effects and emergence of sulfa-resistant strains of the fungus have been reported [6,7]. Thus, up to now pentamidine (1, Figure 1) has emerged as an alternative to treat moderate to severe Pneumocystis-induced pneumonia [2,8]. That molecule exhibits a broad spectrum of antiprotozoal activity: it inhibits the metabolism of p-aminobenzoic acid, interferes with anaerobic glycolysis, acts on oxidative phosphorylation, and impairs nucleic acid and protein synthesis [4]. However administration of pentamidine can be followed by numerous side effects [2,9], including immediate reactions (hypoglycemia, nausea, and tachycardia), local reactions (pain, abscess, or necrosis at the sites of injection), and systemic reactions (nephrotoxicity, leucopenia, abnormalities in glucose metabolism).

Pentamidine is a bisbenzamidine in which both benzamidine functions are linked to a highly flexible chain through aryl ether moieties. Many structural modifications have been considered for years in the laboratories of Tidwell and Boykin [10,11,12,13,14] in order to design and synthesize novel diamidines with the hope to identify less toxic and more efficient drug candidates. The topic actually benefits from a renewal of interest as illustrated by a series of recent publications [15,16,17,18]. In previous work [19], we had demonstrated that 4,4′-(1,4-piperazinediyl)bisbenzamidine (2, Figure 1) was a promising hit characterized by a marked in vitro activity against P. carinii. Moreover, we showed [20] that 3 (Figure 1), the monoamidine analogue of 2, exhibited also an interesting anti-Pneumocystis activity, even at submicromolar concentrations. We now extend those studies by evaluating the effect of compounds bearing amino groups (compounds 4, 5) instead of amidines and derivatives in which the central rigid piperazine has been replaced by a flexible ethylenediamine (compounds 6, 7) or N-methyl ethylenediamine (compounds 8, 9) linkers.

Figure 1. Structure of pentamidine 1 and derivatives 29 considered in this study.
Figure 1. Structure of pentamidine 1 and derivatives 29 considered in this study.
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2. Experimental

2.1. Chemistry

All compounds were prepared according to previously described procedures [15,19,20]. Their structure was determined on the basis of their IR (Perkin-Elmer FTIR 1760 K) and NMR (Bruker AMX 300 MHz) spectra. Identity and purity of novel compounds were in addition confirmed by HRMS (Waters QToF 2) and elemental analyses (Centre Wallon de Recherches Agronomiques, Libramont-Chevigny, Belgium or Laboratoire de Microanalyse Organique of the Institut des Sciences Appliquées de Rouen, France). Derivatives 2 [19], 3 [20], 4 [21], 6 [22], 8 [22], 10 [23], 11 [24], 12 [25], 13 [22], 14 [22], 15 [22], and 17 [26] have been described in the literature. Compound 16 was commercially available (Aldrich, St. Louis, MO, USA).

1-(4-Aminophenyl)-4-phenylpiperazine (5). Yield: 57%. White solid. Mp: 146–149 °C. 1H-NMR (DMSO-d6): δ (ppm): 7.23 (2H, t, J = 8 Hz); 7.02 (2H, d, J = 8 Hz); 6.82 (1H, t, J = 8 Hz); 6.75 (2H, d, J = 9 Hz); 6.51 (2H, d, J = 9 Hz); 4.59 (2H, s, Ar-NH2); 3.24 (4H, t, J = 5 Hz, CH2-N); 3.04 (4H, t, J = 5 Hz, CH2-N). IR (KBr): ν (cm−1): 3359 (N-H), 3220 (N-H), 1600, 1519, 1499. HRMS (ESI-ToF) MH+ C16H21N4: exp: m/z 254.1651; calc.: m/z 254.1657. Elemental analysis C16H19N3: calc.: C 75.85; H 7.56; N 16.59; exp.: C 75.80; H 7.61; N 16.72.

N-(Benzamidine-4-yl)-N′-phenylethane-1,2-diamine sulfate salt (7). Yield: 28%. White solid. Mp: 191–193 °C. 1H-NMR (DMSO-d6): δ (ppm): 8.92 (2H, s, NH amidine); 8.48 (2H, s, NH amidine); 7.71 (2H, d, J = 9 Hz); 7.48 (5H, m); 6.72 (2H, d, J = 9 Hz); 3.54 (4H, s, CH2-NH-Ar and Ph-NH-CH2). IR (KBr): ν (cm−1): 3356 (N-H), 3311 (N-H), 1687, 1464, 1215, 853. HRMS (ESI-ToF) MH+ C16H18N3: exp: m/z 255.1612; calc.: m/z 255.1610. Elemental analysis C15H18N4.1.1H2SO4: calc.: C 49.75; H 5.62; N 15.47; exp.: C 49.49; H 5.70; N 15.49.

N-(Benzamidine-4-yl)-N-methyl-N′-phenylethane-1,2-diamine hydrochloride salt (9). Yield: 30%. White solid. Mp: 212–215 °C. 1H-NMR (DMSO-d6): δ (ppm): 8.87 (2H, s, NH amidine); 8.72 (2H, s, NH amidine); 7.81 (2H, d, J = 9 Hz); 7.07 (2H, t, J = 7 Hz); 6.85 (2H, d, J = 9 Hz); 6.57 (2H, d, J = 7 Hz); 6.50 (1H, t, J = 7 Hz); 5.74 (1H, t br., C6H5-NH); 3.58 (2H, t, J = 5 Hz, CH2-N(CH3)-Ar); 3.21 (2H, t, J = 5 Hz, Ph-NH-CH2); 3.06 (3H, s, CH2-N(CH3)-Ar). IR (KBr): ν (cm−1): 3289 (N-H), 3123 (N-H), 1661, 1600, 1494, 1390, 1178. HRMS (ESI-ToF) MH+ C16H21N4: exp: m/z 269.1762; calc.: m/z 269.1766. Elemental analysis C16H20N4.HCl: calc.: C 63.05; H 6.94; N 18.38; exp.: C 62.72; H 6.93; N 18.45.

N-(4-Cyanophenyl)-N′-phenylethane-1,2-diamine (18). Yield: 63%. White solid. Mp: 101–104 °C. 1H-NMR (DMSO-d6): δ (ppm): 7.52 (2H, d, J = 8 Hz); 7.04 (2H, t, J = 7Hz); 6.83 (1H, t br., NH-ArCN); 6.75 (2H, d, J = 8 Hz); 6.65 (2H, d, J = 7 Hz); 6.52 (1H, t, J = 7 Hz); 5.63 (1H, t br., C6H5-NH); 3.27 (2H, t, J = 5 Hz, CH2-NH-Ar-CN); 3.20 (2H, t, J = 5 Hz, C6H5-NH-CH2). IR (KBr): ν (cm−1): 3419 (N-H), 3362 (N-H), 2210 (C≡N), 1603, 1528, 1172. HRMS (ESI-ToF): MH+ C15H16N3; exp: m/z 238.1342; calc.: m/z 238.1344. Elemental analysis C15H15N3: calc.: C 75.92; H 6.37; N 17.71; exp.: C 75.61; H 6.17; N 17.69.

N-(4-Cyanophenyl)-N-methyl-N′-phenylethane-1,2-diamine (19). Yield: 30%. White solid. Mp: 92–94 °C. 1H-NMR (DMSO-d6): δ (ppm): 7.53 (2H, d, J = 8 Hz); 7.12 (2H, t, J = 7 Hz); 6.85 (2H, d, J = 8 Hz); 6.57 (2H, d, J = 7 Hz); 6.49 (1H, t, J = 7 Hz); 5.61 (1H, br. t, C6H5-NH); 3.58 (2H, t, J = 5 Hz, CH2-N(CH3)-Ar-CN); 3.24 (2H, t, J = 5 Hz, C6H5-NH-CH2); 3.01 (3H, s, CH2- N(CH3)-Ar-CN). IR (KBr): ν (cm−1): 3394 (N-H), 2210 (C≡N), 1609, 1530, 1516, 1385, 807. HRMS (ESI-ToF): MH+ C16H18N3: exp: m/z 252.1498; calc.: m/z 252.1501. Elemental analysis C16H17N3: calc.: C 76.46; H 6.82; N 16.72; exp.: C 76.42; H 6.86; N 16.68.

2.2. Determination of the in Vitro Cytotoxicity

In vitro cytotoxicity assays with established cell lines are useful tools for the general screening of chemicals in toxicological studies [27]. Thus the cytotoxic effect of the compounds has been evaluated by quantification of cell viability of an adherent 3T6 fibroblast cell line (In vitro toxicology assay kit, MTT based, Sigma Aldrich, Bornem, Belgium). The procedure, according to the manufacturer recommendations, was based on the colorimetric determination of the succinate dehydrogenase activity by visualization (540 nm) of the conversion of a tetrazolium salt into formazan [28]. With a microplate reader (Thermo Labsystems Multiskan Ascent 354, Waltham, MA, USA), the optical density of each well was measured at 540 nm against the background absorbance whose reference filter was set at 690 nm. Blank controls (culture medium), free-drug controls, and solvent (DMSO) controls were included in each assay. All cytotoxicity assays were set up in triplicate. The % inhibition of cell proliferation (% IC) was calculated for each compound using the formula: % IC = 100 − [corrected mean OD sample X 100/corrected mean OD solvent controls] where corrected mean OD = mean OD − mean OD of blank controls. The IC50 parameters were obtained by plotting % inhibition values against the logarithm of concentration for each compound.

2.3. Source of P. Carinii

Athymic Pneumocystis-free Lou nu/nu rats (Institut Pasteur de Lille, Lille, France) were used as source of P. carinii organisms for all experiments [29]. Dexamethasone (Merck Sharp & Dohme Chibret, France) was administered to ten-week-old female or male nude rats for two weeks in the drinking water (1 mg/L). Then rats were inoculated with 107 of cryopreserved parasites using a non-surgical endotracheal method [30,31,32,33]. Dexamethasone treatment was maintained until the end of the experiment. Six to eight weeks post-inoculation (p.i.) rats were highly infected without secondary fungal or bacterial infection. Animals were housed in HEPA-filtered air isolators (Flufrance, Val de Reuil, France) and were fed with sterile irradiated food (Scientific Animal Food & Engineering [SAFE], Augy, France) and sterile water ad libitum.

2.4. Extraction, Purification, and Quantification of P. Carinii

Six to eight weeks following inoculation, rats were euthanatized and parasite extraction was performed as previously described [34]. Briefly [34,35], parasites were extracted in Dulbecco's Modified Eagle's Medium (DMEM; BioWhittaker, Hyères, France) by agitation of lung pieces with a magnetic stirrer. The resulting homogenate was poured successively through gauze, 250 and 63 µm stainless steels filters. After centrifugation, the pellet was resuspended in a haemolytic buffered solution. P. carinii organisms were collected by centrifugation and then purified on a polysucrose gradient (Histopaque-1077, Sigma-Aldrich). Blood and Sabouraud dextrose agar (Difco, Pessac, France) media were inoculated with purified parasites to check for the presence of contaminating pathogens. P. carinii was quantitated on air dried smears stained with RAL-555 (Réactifs RAL, Martillac, France), a rapid panoptic methanol-Giemsa-like stain, which stains trophic forms, sporocytes, and cysts [30,35,36]. P. carinii was then cryopreserved by placing parasites in fetal calf serum with 10% dimethyl sulfoxide (DMSO) at −80 °C in a Nalgene 1 °C cryo freezing container (cooling rate: about 1 °C/min) for 4 hours [37]. The parasite samples were then stored in liquid nitrogen. Cryopreserved P. carinii were used for in vitro and in vivo studies.

2.5. Determination of the in Vitro Pneumocystis Activity

Drug stock solutions in DMSO (10 mg/mL) were diluted in Dulbecco's Modified Eagle's Medium (DMEM, BioWhittaker) supplemented with 10% heat-inactivated foetal calf serum (FCS, Gibco-BRL) to produce the required drug concentrations. P. carinii axenic short cultures were performed as follows. All the experiments were carried out in 24-well plates with a final volume of 2 mL of DMEM supplemented with 10% of heat-inactivated fetal calf serum (FCS) containing a final inoculum of 106 organisms per mL. Plates were incubated for 4 days in an atmosphere of 5% CO2 at 37 °C. Then, P. carinii organisms were collected and quantified after RAL-555 panoptic staining. In parallel with these free-drug growth control wells, the anti-Pneumocystis effect of pentamidine and the other bisbenzamidines considered in this study was investigated in the same conditions. All molecules have been screened at a high (50 µg/mL), a medium (10 µg/mL), and a low (0.1 µg/mL) concentration. All experiments were performed in triplicate and data were statistically processed using Student’s t-test. Two-sided p-values < 0.05 were deemed to be statistically significant.

The concentration-effect relationship was established for 1, 6, and 7 by using the Hill equation: ER = ER,max X CS/[(EC50)S + CS] where ER is the effect of each drug concentration (C) on the percentage of inhibition estimated from experimental results; S is a parameter reflecting the steepness of the concentration-effect relationship curve; EC50 is the concentration of the compound at which 50% of the maximum effect (ER,max) is obtained. The parameters of this pharmacodynamic model were calculated by nonlinear least-squares regression techniques using commercial software (Sigma Plot, Systat Software Inc., San Jose, CA, USA).

2.6. Determination of the in Vivo Pneumocystis Activity

All animal experiments were performed following the guidelines of the Institut Pasteur de Lille animal study board, which conforms to the Amsterdam Protocol on animal protection and welfare, and Directive 86/609/EEC on the Protection of Animals Used for Experimental and Other Scientific Purposes, updated in the Council of Europe’s Appendix A ( Treaty/EN/Treaties/PDF/123-Arev.pdf). The animal work also complied with the French law (nu 87-848 dated 19-10-1987) and the European Communities Amendment of Cruelty to Animals Act 1976. All experimental protocols involving animals were carried out by qualified personnel. The animal house (accreditation number: A59107, agreement number: B 59-350009) was placed under the direct control of the director of the Institut Pasteur de Lille who is the “designated responsible person” under French law. The study has been approved by the Ethical Committee for experiments on animals of the region Nord-Pas-de-Calais (approval number CEEA 022011).

To evaluate the in vivo anti-Pneumocystis activity, drug stock solutions in DMSO (300 mg/mL) were diluted in phosphate buffered saline (PBS, BioWhittaker) to produce the required final drug concentrations just before subcutaneous injection. Seven weeks p.i., animals were divided into groups of 4, and then pentamidine and derivatives 4 and 6 were dosed at 5 or 20 mg/kg by subcutaneous route. The drugs were given once a day for 10 consecutive days. The final concentration of DMSO in diluted drug solutions was maintained between 1.5% and 6%. Control animals received doses of 6%-DMSO in sterile water. Twenty-four hours after the end of the treatment, animals were euthanatized and the lung homogenised in a Stomacher-400 blender as previously described [37]. Quantitation of trophic forms, sporocytes, and cysts was performed on air-dried smears stained with RAL-555 stain [34,37,38]. Therapeutic efficacy was assessed by counting P. carinii parasites in lung homogenates and comparing them with those of the untreated controls at the end of the experiment. The results were expressed as percentage of inhibition versus drug-free animal controls and presented as mean and standard error. Data were analyzed using a two-way analysis of variance (PROC MIXED, SAS Institute, Cary, NC, USA), with sex, drug, and their interaction as independent predictors. Due to heterogeneity in parasites count distributions, the first step of the analysis was the selection of the within-subject covariance, based either on likelihood ratio tests or on the Bayesian Information Criterion. Then, tests of the fixed effects were performed. No interaction was found between sex and drug. Therapeutic efficacy was assessed with polynomial contrasts. Regression underlying assumptions were visually inspected with residual plots. Statistical significance was set at α = 0.05.

3. Results and Discussion

3.1. Chemistry

All compounds have been prepared according to established strategies depicted in Scheme 1, Scheme 2, Scheme 3. Reaction of piperazine or 4-phenylpiperazine with 4-fluoronitrobenzene afforded (Scheme 1) the corresponding nitro derivatives 10 and 11 (90% and 81% yield respectively) which were reduced to the amino compounds 4 (59% yield) and 5 (57% yield).

Scheme 1. Procedure for the synthesis of the amine derivatives 4 and 5.
Scheme 1. Procedure for the synthesis of the amine derivatives 4 and 5.
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Three steps were required to synthesize the bisbenzamidines 6 and 8 (Scheme 2). The first one was common to both substances and consisted in the nucleophilic displacement of the fluorine atom in 4-fluorobenzonitrile by the amine function of an ethylenediamine derivative (70% yield for 11 and 12). Depending on the nature of the central linker, the targeted amidines 6 (55% overall yield from 11) and 8 (36% overall yield from 12) were obtained either through reduction by ammonium formate in the presence of Pd/C of the amidoxime 14 or by a Pinner reaction.

Scheme 2. Procedures for the synthesis of bisbenzamidines 6 and 8.
Scheme 2. Procedures for the synthesis of bisbenzamidines 6 and 8.
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Monobenzamidines 7 (36% yield from 16) and 9 (10% yield from 17) have been synthesized in a similar way (Scheme 3). In the case of the preparation of 9, the precursor 17 has been obtained (10% overall yield), by converting N-phenylethanolamine into N-(2-chloroethyl)aniline with thionyl chloride, and further treatment with a methanolic solution of methylamine.

Scheme 3. Procedures for the synthesis of the monobenzamidines 7 and 9.
Scheme 3. Procedures for the synthesis of the monobenzamidines 7 and 9.
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3.2. In Vitro Screenings

Cytotoxicity of derivatives 19 on the 3T6 murine embryonal fibroblast cell line was determined. For each compound, the % inhibition of activity against the concentration scale was plotted. The 50% inhibiting concentrations of cell proliferation (IC50) were calculated by locating the x-axis values corresponding to one-half of the absorbance values. The experiments were repeated in triplicate for each substance. Mean values are reported in Table 1. They ranged from 1.98 (1 and 2) to 56.06 µM (3). Interestingly, the diamidine 6 and the monoamidine 7 were significantly less toxic than pentamidine with IC50 values respectively 15- and 20-fold higher than for the reference compound. It should also be pointed out that N-methylation of the ethylenediamine linker afforded derivatives (8, 9) characterized by a cytotoxicity similar to that of pentamidine.

In a first step the anti-Pneumocystis carinii susceptibility was determined at three concentrations: 50, 10, and 0.1 µg/mL. Derivatives bearing an amine function (compounds 4 and 5) instead of an amidine were inactive even at the highest concentration (50 µg/mL i.e., approximately 200 µM) . Despite the small size of the studied library, this confirms [19] the importance of the amidine moiety to express the antifungal activity. At the lowest concentration, 0.1 µg/mL i.e., 0.17 µM, the reference drug, pentamidine 1, was the most active compound still enabling to inhibit the fungus growth up to a level of 89%. Our data, again [39], emphasized the dramatic influence of the structure of the central linker separating the phamacophores. In fact, replacing the rigid central piperazine spacer in 2 and 3 by a flexible ethylenediamine chain (as in 6 and 7) led to a significant increase of the anti-Pneumocystis effect, whichever the substance is a diamidine whether a monoamidine. Indeed at a concentration of 0.1 µg/mL (i.e., in the range of 0.3 µM for the concerned substances), the piperazine-linked compounds 2 and 3 were less active than the corresponding linear derivatives 6 and 7. On the other hand N-substitution in the ethylenediamine linker (to yield 8 and 9) appeared to be detrimental and even rendered the monoamidine 9 inactive. As mentioned above, those two derivatives exhibited in addition a noticeable cytotoxicity towards the 3T6 cell line.

Table 1. Results of the in vitro cytotoxicity and anti-Pneumocystis screenings for derivatives 19.
Table 1. Results of the in vitro cytotoxicity and anti-Pneumocystis screenings for derivatives 19.
CompoundCytotoxicity 3T6Anti-Pneumocystis activity % inhibition vs. free-drug control at
IC50 (µM)50 µg/mL10 µg/mL0.1 µg/mL*
1 (pentamidine)1.98 ± 0.2099.0 ± 0.199.0 ± 0.188.9 ± 3.1
21.98 ±0.0799.0 ± 0.199.0 ± 0.11.7 ± 2.9
356.06 ± 0.3999.0 ± 0.199.0 ± 0.18.3 ± 14.4
440.8 ± 4.4inactiveinactiveinactive
510.2 ± 0.6inactiveinactiveinactive
630.24 ± 1.1498.7 ± 0.697.7 ± 0.629.8 ± 19.7
740.60 ± 1.3099.5 ± 0.498.7 ± 0.642.0 ± 12.5
82.48 ± 0.3599.0 ± 0.099.0 ± 0.015.0 ± 12.5
93.11 ± 0.0988.7 ± 2.586.3 ± 4.0inactive

* 0.1 µg/mL corresponds to 0.17 (1); 0.26 (2); 0.32 (3); 0.27 (6); 0.28 (7); 0.26 (8); 0.33 (9) µM.

Previous results [34] indicated that in vitro EC50 determination could be a valuable predictive indicator of the in vivo anti-Pneumocystis carinii pneumonia effect of a drug candidate. Therefore we selected pentamidine 1 and the two most active derivatives, 6 and 7, to establish concentration-effect curves. Incubation time was fixed to 4 days and final drug concentrations range from 150 to 1.35 10−5 µM. Inspection of the results gathered in Figure 2 confirms the preliminary observations. Pentamidine 1 was the most potent drug with an EC50 value of 0.08 ± 0.01 µM (p < 0.05), followed by the monoamidine 7 (EC50 = 0.40 ± 0.08 µM, p < 0.05) and the diamidine 6 (EC50 = 0.60 ± 0.12 µM, p < 0.05). In terms of efficacy, all three derivatives exhibited similar optimum Emax values of 99.37 ± 2.54 µM (7), 99.51 ± 3.78 µM (1), and 101.24 ± 3.90 µM (6). In terms of concentration-effect relationships the steepness of the curves showed slight differences: S = 1.09 ± 0.23 for 1, 1.49 ± 0.31 for 6, and 2.00 ± 0.54 for 7. Therefore the monoamidine 7 should be the most sensible to variations of concentrations.

3.3. In Vivo Study

An in vivo experiment was performed in order to explore whether it reflected in vitro results. The used in vivo model was the athymic Pneumocystis-free Lou nu/nu rats non-surgically inoculated by endo-tracheal route [29,30] with a suspension of P. carinii organisms [32]. This model did not present the drawbacks of conventional corticosteroid-induced PcP rodent models [30], which are still used however. Rats developed high infection levels 6–8 weeks post inoculation without secondary fungal or bacterial infection. The non-surgical endotracheal inoculation method revealed to be simple, reliable, reproducible, and animal welfare compliant. Thus animals were endotracheally infected after 2 weeks of dexamethasone administration (1 mg/L, drinking water) with 107 P. carinii organisms. Drugs were administered once a day by subcutaneous route for 10 consecutive days. Untreated animals developed extensive infections at the end of the treatment period. The number of total P. carinii organisms per lung in DMSO control rats was 1.26 ± 0.20 × 1010. As illustrated in Figure 3, derivative 6 showed a strong anti-Pneumocystis activity comparable to that of pentamidine at both doses. On the other hand, whereas showing a strong anti-Pneumocystis activity in vitro, the monoamidine 7 appeared to be poorly potent when administered subcutaneously to Pneumocystis-infected rats, even at the dose of 20 mg/kg/day. That observation could be related to results [40] suggesting that the monoamidine 7 is more rapidly eliminated from plasma in comparison with pentamidine and the diamidine 6.

Figure 2. Concentration-in vitro activity relationships of pentamidine (1) and derivatives 6 and 7 against P. carinii (results were calculated after 4 days of culture).
Figure 2. Concentration-in vitro activity relationships of pentamidine (1) and derivatives 6 and 7 against P. carinii (results were calculated after 4 days of culture).
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Figure 3. Therapeutic efficacy of pentamidine (1) and derivatives 6 and 7 against experimental Pneumocystis carinii pneumonia in nude rats (the numbers at the top of each bar graph indicate the % of inhibition versus control rats).
Figure 3. Therapeutic efficacy of pentamidine (1) and derivatives 6 and 7 against experimental Pneumocystis carinii pneumonia in nude rats (the numbers at the top of each bar graph indicate the % of inhibition versus control rats).
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4. Conclusions

Previous work [29] identified 4,4′-(1,4-piperazinediyl)bisbenzamidine (2) as an interesting anti-Pneumocystis carinii agent. We now have demonstrated that replacing the piperazine moiety by a flexible ethylenediamine chain yielded a novel promising hit, namely N,N′-bis(benzamidine-4-yl)ethane-1,2-diamine (6), characterized by a significant efficacy against P. carinii in vitro as well as in vivo. That derivative 6 exhibited a lower in vitro toxicity than pentamidine and was more efficient in vivo than the corresponding monoamidine 7. Visual observations also indicated that rats treated with 6 maintained a quite normal behavior during the treatment and, last but not least, those rats did not develop any cutaneous reaction at the site of injections whereas deleterious wounds could be seen on the skin of the animals treated with pentamidine (data not shown).

Although genomic and phenotypic host species-related differences have been reported among Pneumocystis species, yielding a strong host-species specificity [1,36], it has not been established whether Pneumocystis species from different mammal hosts can exhibit different drug susceptibility patterns. The topic was rarely addressed but until now results obtained with P. carinii in vitro or in vivo could be extrapolated successfully to P. jirovecii in humans [41]. Therefore, 6, the hit identified in this work, deserves further studies, especially as a potential candidate for the treatment of Pneumocystis carinii pneumonia cases.


D.S. and J.J.V.E. are grateful to the Belgian Fonds National de la Recherche Scientifique for a financial support.

Conflict of Interest

The authors declare no conflict of interest.


  1. Chabé, M.; Aliouat-Denis, C.; Delhaes, L.; Aliouat, E-M.; Viscogliosi, E.; Dei-Cas, E. Pneumocystis: From a doubtful unique entity to a group of highly diversified fungal species. FEMS Yeast Res. 2011, 11, 2–17. [Google Scholar] [CrossRef]
  2. Calderon, E.J.; Gutierrez-Rivero, S.; Durand-Joly, I.; Dei-Cas, E. Pneumocystis infection in humans: Diagnosis and treatment. Expert Rev. Anti-Infect. Ther. 2010, 8, 683–701. [Google Scholar] [CrossRef]
  3. Huang, L. Clinical and translational research in Pneumocystis pneumonia. Parasite 2011, 18, 3–11. [Google Scholar] [CrossRef]
  4. Larsen, H.H.; von Linstow, M.L.; Lundgren, B.; Hogh, B.; Westh, H.; Lundgren, J.D. Primary Pneumocystis infection in infants hospitalized with acute respiratory tract infection. Emerg. Infect. Dis. 2007, 13, 66–72. [Google Scholar]
  5. Carmona, E.M.; Limper, A.H. Update on the diagnosis and treatment of Pneumocystis pneumonia. Ther. Adv. Respir. Dis. 2011, 5, 41–59. [Google Scholar] [CrossRef]
  6. Iliades, P.; Meshnick, S.R.; Macreadie, I.G. Dihydropteroate synthase mutations in Pneumocystis jiroveci can affect sulfamethoxazole resistance in a Saccharomyces cerevisiae model. Antimicrob. Agents Chemother. 2004, 48, 2617–2623. [Google Scholar]
  7. Friaza, V.; Montes-Cano, M.A.; Respaldiza, N.; Morilla, R.; Calderon, E.J.; de la Horra, C. Prevalence of dihyropteroate synthase mutations in Spanish patients with HIV-associated Pneumocystis pneumonia. Diagn. Microbiol. Infect. Dis. 2009, 64, 104–105. [Google Scholar] [CrossRef]
  8. Catherinot, F.; Bougnoux, M.E.; Lecuit, M.; Couderc, L.J.; Lortholary, O. Pneumocystis jiroveci pneumonia. Infect. Dis. Clin. North Am. 2010, 24, 107–138. [Google Scholar] [CrossRef]
  9. Huang, L.; Morris, A.; Limper, A.H.; Beck, J.M. ATS Pneumocystis Workshop Participants, An Official ATS Workshop Summary: Recent advances and future directions in Pneumocystis pneumonia (PCP). Proc. Am. Thorac. Soc. 2006, 3, 655–664. [Google Scholar] [CrossRef]
  10. Tidwell, R.R.; Jones, S.K.; Geratz, J.D.; Ohemeng, K.A.; Cory, M.; Hall, J.E. Analogs of 1,5-di(4-amidinophenoxy)pentane (pentamidine) in the treatment of experimental Pneumocystis carinii pneumonia. J. Med. Chem. 1990, 33, 1252–1257. [Google Scholar] [CrossRef]
  11. Jones, S.K.; Hall, J.E.; Allen, M.A.; Morrison, S.D.; Ohemeng, K.A.; Reddy, V.V.; Geratz, J.E.; Tidwell, R.R. Novel pentamidine analogs in the treatment of experimental Pneumocystis carinii pneumonia. Antimicrob. Agents Chemother. 1990, 34, 1026–1030. [Google Scholar] [CrossRef]
  12. Patrick, D.A.; Boykin, D.W.; Wilson, W.D.; Tanous, F.A.; Spychala, J.; Bender, B.C.; Hall, J.E.; Dykstra, C.C.; Ohemeng, K.A.; Tidwell, R.R. Anti-Pneumocystis carinii pneumonia activity of dicationic carbazoles. Eur. J. Med. Chem. 1997, 32, 781–793. [Google Scholar] [CrossRef]
  13. Boykin, D.W.; Kumar, A.; Bajic, M.; Xiao, G.; Wilson, W.D.; Bender, B.C.; McCurdy, D.R.; Hall, J.E.; Tidwell, R.R. Anti-Pneumocystis activity of dicationic diaryl methylpyrimidines. Eur. J. Med. Chem. 1997, 32, 965–972. [Google Scholar]
  14. Francesconi, I.; Wilson, W.D.; Tanious, F.A.; Hall, J.E.; Bender, B.C.; Tidwell, R.R.; McCurdy, D.; Boykin, D.W. 2,4-Diphenyl furan diamidines as novel anti-Pneumocystis carinii pneumonia agents. J. Med. Chem. 1999, 42, 2260–2265. [Google Scholar] [CrossRef]
  15. Huang, T.L.; Vanden Eynde, J.J.; Mayence, A.; Collins, M.S.; Cushion, M.T.; Rattendi, D.; Londono, I.; Mazumder, L.; Bacchi, C.J.; Yartlet, N. Synthesis and SAR of alkanediamide-linked bisbenzamidines with anti-trypanosomal and anti-Pneumocystis activity. Bioorg. Med. Chem. Lett. 2009, 19, 5884–5886. [Google Scholar] [CrossRef]
  16. Bakunov, S.A.; Bakunova, S.M.; Wenzler, T.; Ghebru, M.; Werbovetz, K.A.; Brun, R.; Tidwell, R.R. Synthesis and antiprotozoal activity of cationic 1,4-diphenyl-1H-1,2,3-triazoles. J. Med. Chem. 2010, 53, 254–272. [Google Scholar]
  17. Farahat, A.A.; Paliakov, E.; Kumar, A.; Barghash, A.E.M.; Goda, F.E.; Eisa, H.M.; Wenzler, T.; Brun, R.; Liu, Y.; Wilson, W.D.; et al. Exploration of larger central ring linker in furamidine analogues: Synthesis and evaluation of their DNA binding, antiparasitic and fluorescence properties. Bioorg. Med. Chem. 2011, 19, 2156–2167. [Google Scholar] [CrossRef]
  18. Maciejewska, D.; Zabinski, J.; Kazmierczak, P.; Rezler, M.; Krassowska-Swiebocka, B.; Collins, M.S.; Cushion, M.T. Analogs of pentamidine as potential anti-Pneumocystis chemotherapeutics. Eur. J. Med. Chem. 2012, 48, 164–173. [Google Scholar] [CrossRef]
  19. Cushion, M.T.; Walzer, P.D.; Collins, M.S.; Rebholz, S.; Vanden Eynde, J.J.; Mayence, A.; Huang, T.L. Highly active anti-Pneumocystis carinii compounds in a library of novel piperazine-linked bisbenzamidines and related compounds. Antimicrob. Agents Chemother. 2004, 48, 4209–4216. [Google Scholar]
  20. Laurent, J.; Stanicki, D.; Huang, T.L.; Dei-Cas, E.; Pottier, M.; Aliouat, E.M.; Vanden Eynde, J.J. Bisbenzamidines as antifungal agents. Are both amidine functions required to observe an anti-Pneumocystis carinii activity? Molecules 2010, 15, 4283–4293. [Google Scholar]
  21. Slifkin, S.C. Light sensitive diazotype materials. US Patent 2632703, 24 March 1953. [Google Scholar]
  22. Vamecq, J.; Maurois, P.; Pages, N.; Bac, P.; Stables, J.P.; Gressens, P.; Stanicki, D.; Vanden Eynde, J.J. 1,2-Ethane-bis-1-amino-4-benzamidine is active against several brain insult and seizure challenges through anti-NMDA mechanisms targeting the 3H-TCP binding site and antioxidant action. Eur. J. Med. Chem. 2010, 45, 3101–3110. [Google Scholar] [CrossRef]
  23. Winkelman, E.; Raether, W.; Duewel, W.; Gericke, D.; Hohorst, W.; Rolly, H.; Schrinner, E. Chemotherapeutisch wirksame Nitroverbindungen. Arzneim. Forsch. 1975, 25, 681–708. [Google Scholar]
  24. Popov, D. Synthesis of 1,4-unsymmetrically substituted piperazines. Preparation of 1-phenyl-4-arylpiperazines. C. R. Acad. Bulg. Sci. 1966, 19, 1663–1666. [Google Scholar]
  25. Berg, S.S. The search for chemotherapeutic amidines. Part XVII. α,ω-Di-p-amidinoanilino-alkanes. J. Chem. Soc. 1968, 5172–5176. [Google Scholar]
  26. Fourneau, J.-P.; de Lestrange, Y. Derivatives of N-phenylethylenediamine. Bull. Soc. Chim. France 1947, 185, 827–838. [Google Scholar]
  27. Crespi, C.I. Xenobiotic-metabolizing human cells as tools for pharmacological and toxicological research. Adv. Drug Res. 1995, 26, 179–235. [Google Scholar] [CrossRef]
  28. Mosmann, T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J. Immunol. Methods 1983, 62, 55–63. [Google Scholar]
  29. Martinez, A.; Aliouat, E.M.; Pottier, M.; Gantois, N.; Pinçon, C.; Standaert-Vitse, A.; Dei-Cas, E.; Aliouat-Denis, C.-M. High-speed cell sorting of infectious trophic and cystic forms of Pneumocystis carinii. J. Eukaryot. Microbiol. 2009, 56, 446–453. [Google Scholar] [CrossRef]
  30. Dei-Cas, E.; Fleurisse, L.; Aliouat, E.M.; Bahon-Le-Capon, J.; Cailliez, C.; Creusy, C. Morphological and ultrastructural methods for Pneumocystis. FEMS Immunol. Med. Microbiol. 1998, 22, 185–189. [Google Scholar] [CrossRef]
  31. Garry, S.; Nesslany, F.; Aliouat, E.M.; Haguenoer, J.M.; Marzin, D. Hematite (Fe(2)O(3)) enhances benzo[a]pyrene genotoxicity in endotracheally treated rat, as determined by Comet Assay. Mutation Res. 2003, 538, 19–29. [Google Scholar]
  32. Ambrose, H.E.; Keely, S.P.; Aliouat, E.M.; Dei-Cas, E.; Wakefield, A.E.; Miller, R.F.; Stringer, J.R. Expression of the MSG and PRT1 multigene families of Pneumocystis carinii are not regulated in the same manner. Microbiology 2004, 150, 293–300. [Google Scholar] [CrossRef]
  33. Smith, W.; Bartlett, M.S.; Queener, S.F. Development of models and their use to discover new drugs for therapy and prophylaxis of Pneumocystis carinii Pneumonia. In Pneumocystis, Pneumonia, 3rd ed.; Walzer, P.D., Cushion, M.T., Eds.; Marcel Dekker, Inc.: New York, NY, USA, 2004; pp. 487–509. [Google Scholar]
  34. Aviles, P.; Aliouat, E.M.; Martinez, A.; Dei-Cas, E.; Herreros, E.; Dujardin, L.; Gargallo-Viola, D. In vitro pharmacodynamic parameters of sordarin derivatives in comparison with atovaquone, pentamidine and trimethoprim/sulfamethoxazol against rat-derived Pneumocystis carinii. Antimicrob. Agents Chemother. 2000, 44, 1284–1290. [Google Scholar] [CrossRef]
  35. Aliouat, E.M.; Dujardin, L.; Martínez, A.; Duriez, T.; Dei-Cas, E. Pneumocystis carinii growth kinetics in culture systems and in hosts: involvement of each life cycle parasite stage. J. Eukaryot. Microbiol. 1999, 46, 116S–117S. [Google Scholar] [CrossRef]
  36. Dei-Cas, E.; Chabé, M.; Moukhlis, R.; Durand-Joly, I.; Aliouat, E.M.; Stringer, J.R.; Cushion, M.T.; Noel, C.; de Hoog, G.S.; Guillot, J.; Viscogliosi, E. Pneumocystis oryctolagi sp. nov., an uncultured fungus causing pneumonia in rabbits at weaning: Review of current knowledge, and description of a new taxon on genotypic, phylogenetic and phenotypic bases. FEMS Microbiol. Rev. 2006, 30, 853–871. [Google Scholar]
  37. Dei-Cas, E.; Cailliez, J.C. European Concerted Action on Pneumocystis carinii. In vitro systems in Pneumocystis research. Parasitol. Today 1996, 12, 245–249. [Google Scholar] [CrossRef]
  38. Dei-Cas, E.; Aliouat, E.M.; Cailliez, J.C. Cellular Structure. In Pneumocystis Pneumonia, 3rd ed.; Walzer, P.D., Cushion, M.T., Eds.; Marcel Dekker, Inc.: New York, NY, USA, 2004; pp. 61–94. [Google Scholar]
  39. Tao, B.; Huang, T.L.; Zhang, Q.; Jackson, L.; Queener, S.F.; Donkor, I.O. Synthesis and anti-Pneumocystis carinii activity of conformationally restricted analogues of pentamidine. Eur. J. Med. Chem. 1999, 34, 531–538. [Google Scholar] [CrossRef]
  40. Hambye, S.; Stanicki, D.; Colet, J.M.; Aliouat, E.M.; Vanden Eynde, J.J.; Blankert, B. Three optimized and validated (using accuracy profiles) LC methods for the determination of pentamidine and new analogs in rat plasma. Talanta 2011, 83, 832–839. [Google Scholar] [CrossRef]
  41. Hughes, W.T.; Killmar, J.T.; Oz, H.S. Relative potency of 10 drugs with anti-Pneumocystis carinii activity in an animal model. J. Infect. Dis. 1994, 170, 906–911. [Google Scholar]
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