Poly(ethylene glycol)-Lipid-Conjugated Antibodies Enhance Dendritic Cell Phagocytosis of Apoptotic Cancer Cells
Abstract
:1. Introduction
2. Experimental Section
2.1. Chemicals, Antibodies and Cell-Lines
2.2. Synthesis of BAM
2.3. Preparation of BAM-Conjugated IgG
2.4. Incorporation of IgG-BAM Conjugates onto Cell Membrane
2.5. Generation of Bone Marrow-derived DCs
2.6. Phagocytosis of Apoptotic Cancer Cells by DCs
3. Results and Discussion
3.1. Conjugation of IgG and BAM
3.2. Incorporation of IgG-BAM Conjugates onto the Cell Membrane
3.3. Effects of Incorporated IgG-BAM on Phagocytosis by Dendritic Cells
4. Conclusions
Acknowledgements
References
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Tomita, U.; Yamaguchi, S.; Sugimoto, Y.; Takamori, S.; Nagamune, T. Poly(ethylene glycol)-Lipid-Conjugated Antibodies Enhance Dendritic Cell Phagocytosis of Apoptotic Cancer Cells. Pharmaceuticals 2012, 5, 405-416. https://doi.org/10.3390/ph5050405
Tomita U, Yamaguchi S, Sugimoto Y, Takamori S, Nagamune T. Poly(ethylene glycol)-Lipid-Conjugated Antibodies Enhance Dendritic Cell Phagocytosis of Apoptotic Cancer Cells. Pharmaceuticals. 2012; 5(5):405-416. https://doi.org/10.3390/ph5050405
Chicago/Turabian StyleTomita, Urara, Satoshi Yamaguchi, Yoichiro Sugimoto, Satoshi Takamori, and Teruyuki Nagamune. 2012. "Poly(ethylene glycol)-Lipid-Conjugated Antibodies Enhance Dendritic Cell Phagocytosis of Apoptotic Cancer Cells" Pharmaceuticals 5, no. 5: 405-416. https://doi.org/10.3390/ph5050405