Lecithin and Chitosan as Building Blocks in Anti-Candida Clotrimazole Nanoparticles
Abstract
:1. Introduction
2. Results and Discussion
2.1. Nanoparticle Development
2.2. Nanoparticle Characteristics
2.3. Nanoparticle Stability
2.4. Evaluation of Toxicity
2.5. Anti-Candida Activity
3. Materials and Methods
3.1. Materials
3.2. Nanoparticle Preparation
3.3. Characterisation of Nanoparticles
3.3.1. Size, Zeta Potential, and pH Determination
3.3.2. CLO-LCN Morphology
3.3.3. Nanoparticle Stability
3.4. Toxicity Evaluation
3.5. Anti-Candida Evaluation
3.5.1. Anti-Candida Activity Determined as Zone of Inhibition
3.5.2. Anti-Candida Activity Determined through Broth Microdilution
3.6. Statistical Analyses
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Size 1 (≥75%, nm) | PI 2 | Zeta Potential (mV) | EE% 3 | pH | |
---|---|---|---|---|---|
LCNs | 350 ± 47 | 0.26 ± 0.01 | 31.7 ± 4.0 | - | 4.19 ± 0.07 |
CLO-LCNs | 189 ± 24 | 0.28 ± 0.07 | 24.3 ± 4.0 | 14.7 ± 2.5 | 4.39 ± 0.11 |
Zone of Inhibition | |||
---|---|---|---|
LCNs | Free CLO | CLO-LCNs | |
C. albicans SO1 | N.I. | 4.12 ± 0.37 | 3.83 ± 0.44 |
C. albicans SO2 | N.I. | 7.06 ± 0.57 | 6.79 ± 0.40 |
LCN | CLO-LCNs | |
---|---|---|
Chitosan/lecithin ratio | 1:20 | 1:20 |
CLO | - | 0.82 mg/mL |
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Hemmingsen, L.M.; Panzacchi, V.; Kangu, L.M.; Giordani, B.; Luppi, B.; Škalko-Basnet, N. Lecithin and Chitosan as Building Blocks in Anti-Candida Clotrimazole Nanoparticles. Pharmaceuticals 2023, 16, 790. https://doi.org/10.3390/ph16060790
Hemmingsen LM, Panzacchi V, Kangu LM, Giordani B, Luppi B, Škalko-Basnet N. Lecithin and Chitosan as Building Blocks in Anti-Candida Clotrimazole Nanoparticles. Pharmaceuticals. 2023; 16(6):790. https://doi.org/10.3390/ph16060790
Chicago/Turabian StyleHemmingsen, Lisa Myrseth, Virginia Panzacchi, Lloyd Mbugua Kangu, Barbara Giordani, Barbara Luppi, and Nataša Škalko-Basnet. 2023. "Lecithin and Chitosan as Building Blocks in Anti-Candida Clotrimazole Nanoparticles" Pharmaceuticals 16, no. 6: 790. https://doi.org/10.3390/ph16060790