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• De Stefano, L
• Arcari, P
• Lamberti, A
• Sanges, C
• Rotiroti, L
• Rea, I
• Rendina, I
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• De Stefano, L
• Arcari, P
• Lamberti, A
• Sanges, C
• Rotiroti, L
• Rea, I
• Rendina, I
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• De Stefano, L
• Arcari, P
• Lamberti, A
• Sanges, C
• Rotiroti, L
• Rea, I
• Rendina, I

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Sensors 2007, 7(2), 214-221; doi:10.3390/s7020214

Article

DNA Optical Detection Based on Porous Silicon Technology: from Biosensors to Biochips

Luca De Stefano^1,* , Paolo Arcari² , Annalisa Lamberti² , Carmen Sanges² , Lucia Rotiroti¹ , Ilaria Rea¹  and Ivo Rendina¹

¹ Institute for Microelectronics and Microsystems – Unit of Naples – National Council of Research, Via P. Castellino 111, 80131 Napoli, Italy ² Department of Biochemistry and Medical Biotechnologies, University of Naples “Federico II”, Via S. Pansini 5, 80131 Napoli, Italy ³ Department of Organic Chemistry and Biochemistry, University of Naples “Federico II”, Via Cinthia, 80128 Napoli, Italy ⁴ Department of Physical Sciences, University of Naples “Federico II”, Via Cinthia, 80126 Naples, Italy

* Author to whom correspondence should be addressed.

Received: 15 February 2007; in revised form: 26 February 1995 / Accepted: 1 January 2007 / Published: 28 February 2007

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Abstract: A photochemical functionalization process which passivates the porous silicon surface of optical biosensors has been optimized as a function of the thickness and the porosity of the devices. The surface modification has been characterized by contact angle measurements. Fluorescence measurements have been used to investigate the stability of the DNA single strands bound to the nanostructured material. A dose-response curve for an optical label-free biosensor in the 6-80 μM range has been realized.

Keywords: Optical Biosensors; Porous Silicon; Biochip.

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