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Modelling a Peroxidase-based Optical Biosensor
Department of Software Engineering, Vilnius University, Naugarduko 24, LT-03225 Vilnius, Lithuania
Institute of Mathematics and Informatics, Akademijos 4, LT-08663 Vilnius, Lithuania
Department of Chemistry and Bioengineering, Vilnius Gediminas Technical University, Sauletekio al. 11, LT-10223 Vilnius, Lithuania
* Author to whom correspondence should be addressed.
Received: 8 October 2007; Accepted: 9 November 2007 / Published: 14 November 2007
Abstract: The response of a peroxidase-based optical biosensor was modelled digitally.A mathematical model of the optical biosensor is based on a system of non-linear reaction-diffusion equations. The modelling biosensor comprises two compartments, an enzyme layerand an outer diffusion layer. The digital simulation was carried out using finite differencetechnique. The influence of the substrate concentration as well as of the thickness of both theenzyme and diffusion layers on the biosensor response was investigated. Calculations showedcomplex kinetics of the biosensor response, especially at low concentrations of the peroxidaseand of the hydrogen peroxide.
Keywords: optical biosensor; peroxidase; modelling; simulation.
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MDPI and ACS Style
Baronas, R.; Gaidamauskait˙e, E.; Kulys, J. Modelling a Peroxidase-based Optical Biosensor. Sensors 2007, 7, 2723-2740.
Baronas R, Gaidamauskait˙e E, Kulys J. Modelling a Peroxidase-based Optical Biosensor. Sensors. 2007; 7(11):2723-2740.
Baronas, Romas; Gaidamauskait˙e, Evelina; Kulys, Juozas. 2007. "Modelling a Peroxidase-based Optical Biosensor." Sensors 7, no. 11: 2723-2740.