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Sensors 2007, 7(11), 2723-2740; doi:10.3390/s7112723

Article

Modelling a Peroxidase-based Optical Biosensor

Romas Baronas ^1,* , Evelina Gaidamauskait˙e ¹  and Juozas Kulys ³

¹ Department of Software Engineering, Vilnius University, Naugarduko 24, LT-03225 Vilnius, Lithuania ² Institute of Mathematics and Informatics, Akademijos 4, LT-08663 Vilnius, Lithuania ³ Department of Chemistry and Bioengineering, Vilnius Gediminas Technical University, Sauletekio al. 11, LT-10223 Vilnius, Lithuania

* Author to whom correspondence should be addressed.

Received: 8 October 2007 / Accepted: 9 November 2007 / Published: 14 November 2007

(This article belongs to the Special Issue Optical Biosensors)

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Abstract: The response of a peroxidase-based optical biosensor was modelled digitally.A mathematical model of the optical biosensor is based on a system of non-linear reaction-diffusion equations. The modelling biosensor comprises two compartments, an enzyme layerand an outer diffusion layer. The digital simulation was carried out using finite differencetechnique. The influence of the substrate concentration as well as of the thickness of both theenzyme and diffusion layers on the biosensor response was investigated. Calculations showedcomplex kinetics of the biosensor response, especially at low concentrations of the peroxidaseand of the hydrogen peroxide.

Keywords: optical biosensor; peroxidase; modelling; simulation.

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