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Enhancement of BSA Binding on Au Surfaces by calixbisazacrown Monolayer
College of Pharmacy, Pusan National University, Pusan 609-735, Korea
College of Nanoscience and Nanotechnology, Pusan National University, Pusan 609-735, Korea
College of Engineering, Kyungpook National University, Taegu 702-701, Korea
* Author to whom correspondence should be addressed.
Received: 21 September 2007; Accepted: 10 October 2007 / Published: 11 October 2007
Abstract: Effective investigation of biomolecular structure and function with chip-basedmodern instruments often requires reliable and steady attachment of designatedbiomolecules on substrate. Here, we investigated the formation of self-assembled monolayer(SAM) with a new calixarene derivative containing bisazacrown ether at the lower rim(calixbisazacrown) where ammonium moieties of proteins can mainly be interacted with.Immobilization process of protein using bovine serum albumin (BSA) on the Au surfacemodified with calixbisazacrown monolyer as an artificial linker system was monitored bysurface plasmon resonance (SPR) technique. The surface concentration of BSA calculatedby the simulation of SPR experimental data was higher than that of a well-known similarcommercial protein linker. These results can help in modeling and understanding of proteinimmobilization on solid surface as well as further development lab-on-a-chip (LOC) devicesfor biomedical diagnosis kit of certain protein related diseases as biomarkers.
Keywords: Azacrown ether; Self-assembled monolayer (SAM); Linker system; Bovine serum albumin (BSA); Biochip.
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MDPI and ACS Style
Chen, H.; Kim, Y.S.; Lee, J.; Yoon, S.J.; Lim, D.S.; Choi, H.-J.; Koh, K. Enhancement of BSA Binding on Au Surfaces by calixbisazacrown Monolayer. Sensors 2007, 7, 2263-2272.
Chen H, Kim YS, Lee J, Yoon SJ, Lim DS, Choi H-J, Koh K. Enhancement of BSA Binding on Au Surfaces by calixbisazacrown Monolayer. Sensors. 2007; 7(10):2263-2272.
Chen, Hongxia; Kim, Youn S.; Lee, Jaebeom; Yoon, Seok J.; Lim, Dong S.; Choi, Heung-Jin; Koh, Kwangnak. 2007. "Enhancement of BSA Binding on Au Surfaces by calixbisazacrown Monolayer." Sensors 7, no. 10: 2263-2272.