Sensors 2007, 7(10), 2263-2272; doi:10.3390/s7102263

Enhancement of BSA Binding on Au Surfaces by calix[4]bisazacrown Monolayer

1, 2, 2, 3, 3, 3 and 2,* email
Received: 21 September 2007; Accepted: 10 October 2007 / Published: 11 October 2007
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Effective investigation of biomolecular structure and function with chip-basedmodern instruments often requires reliable and steady attachment of designatedbiomolecules on substrate. Here, we investigated the formation of self-assembled monolayer(SAM) with a new calix[4]arene derivative containing bisazacrown ether at the lower rim(calix[4]bisazacrown) where ammonium moieties of proteins can mainly be interacted with.Immobilization process of protein using bovine serum albumin (BSA) on the Au surfacemodified with calix[4]bisazacrown monolyer as an artificial linker system was monitored bysurface plasmon resonance (SPR) technique. The surface concentration of BSA calculatedby the simulation of SPR experimental data was higher than that of a well-known similarcommercial protein linker. These results can help in modeling and understanding of proteinimmobilization on solid surface as well as further development lab-on-a-chip (LOC) devicesfor biomedical diagnosis kit of certain protein related diseases as biomarkers.
Keywords: Azacrown ether; Self-assembled monolayer (SAM); Linker system; Bovine serum albumin (BSA); Biochip.
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MDPI and ACS Style

Chen, H.; Kim, Y.S.; Lee, J.; Yoon, S.J.; Lim, D.S.; Choi, H.-J.; Koh, K. Enhancement of BSA Binding on Au Surfaces by calix[4]bisazacrown Monolayer. Sensors 2007, 7, 2263-2272.

AMA Style

Chen H, Kim YS, Lee J, Yoon SJ, Lim DS, Choi H-J, Koh K. Enhancement of BSA Binding on Au Surfaces by calix[4]bisazacrown Monolayer. Sensors. 2007; 7(10):2263-2272.

Chicago/Turabian Style

Chen, Hongxia; Kim, Youn S.; Lee, Jaebeom; Yoon, Seok J.; Lim, Dong S.; Choi, Heung-Jin; Koh, Kwangnak. 2007. "Enhancement of BSA Binding on Au Surfaces by calix[4]bisazacrown Monolayer." Sensors 7, no. 10: 2263-2272.

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