Enhancement of BSA Binding on Au Surfaces by calix[4]bisazacrown Monolayer

doi:10.3390/s7102263

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Sensors 2007, 7(10), 2263-2272; doi:10.3390/s7102263

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Enhancement of BSA Binding on Au Surfaces by calix[4]bisazacrown Monolayer

Hongxia Chen¹, Youn Sook Kim², Jaebeom Lee², Seok Ju Yoon³, Dong Seob Lim³, Heung-Jin Choi³ and Kwangnak Koh^2,*

¹ College of Pharmacy, Pusan National University, Pusan 609-735, Korea ² College of Nanoscience and Nanotechnology, Pusan National University, Pusan 609-735, Korea ³ College of Engineering, Kyungpook National University, Taegu 702-701, Korea

* Author to whom correspondence should be addressed.

Received: 21 September 2007 / Accepted: 10 October 2007 / Published: 11 October 2007

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Abstract: Effective investigation of biomolecular structure and function with chip-basedmodern instruments often requires reliable and steady attachment of designatedbiomolecules on substrate. Here, we investigated the formation of self-assembled monolayer(SAM) with a new calix[4]arene derivative containing bisazacrown ether at the lower rim(calix[4]bisazacrown) where ammonium moieties of proteins can mainly be interacted with.Immobilization process of protein using bovine serum albumin (BSA) on the Au surfacemodified with calix[4]bisazacrown monolyer as an artificial linker system was monitored bysurface plasmon resonance (SPR) technique. The surface concentration of BSA calculatedby the simulation of SPR experimental data was higher than that of a well-known similarcommercial protein linker. These results can help in modeling and understanding of proteinimmobilization on solid surface as well as further development lab-on-a-chip (LOC) devicesfor biomedical diagnosis kit of certain protein related diseases as biomarkers.

Keywords: Azacrown ether; Self-assembled monolayer (SAM); Linker system; Bovine serum albumin (BSA); Biochip.

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Cite This Article

MDPI and ACS Style

Chen, H.; Kim, Y.S.; Lee, J.; Yoon, S.J.; Lim, D.S.; Choi, H.-J.; Koh, K. Enhancement of BSA Binding on Au Surfaces by calix[4]bisazacrown Monolayer. Sensors 2007, 7, 2263-2272.

AMA Style

Chen H., Kim Y.S., Lee J., Yoon S.J., Lim D.S., Choi H.-J., Koh K. Enhancement of BSA Binding on Au Surfaces by calix[4]bisazacrown Monolayer. Sensors. 2007; 7(10):2263-2272.

Chicago/Turabian Style

Chen, Hongxia; Kim, Youn S.; Lee, Jaebeom; Yoon, Seok J.; Lim, Dong S.; Choi, Heung-Jin; Koh, Kwangnak. 2007. "Enhancement of BSA Binding on Au Surfaces by calix[4]bisazacrown Monolayer." Sensors 7, no. 10: 2263-2272.

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