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Enhancement of BSA Binding on Au Surfaces by calixbisazacrown Monolayer
AbstractEffective investigation of biomolecular structure and function with chip-basedmodern instruments often requires reliable and steady attachment of designatedbiomolecules on substrate. Here, we investigated the formation of self-assembled monolayer(SAM) with a new calixarene derivative containing bisazacrown ether at the lower rim(calixbisazacrown) where ammonium moieties of proteins can mainly be interacted with.Immobilization process of protein using bovine serum albumin (BSA) on the Au surfacemodified with calixbisazacrown monolyer as an artificial linker system was monitored bysurface plasmon resonance (SPR) technique. The surface concentration of BSA calculatedby the simulation of SPR experimental data was higher than that of a well-known similarcommercial protein linker. These results can help in modeling and understanding of proteinimmobilization on solid surface as well as further development lab-on-a-chip (LOC) devicesfor biomedical diagnosis kit of certain protein related diseases as biomarkers.
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Chen, H.; Kim, Y.S.; Lee, J.; Yoon, S.J.; Lim, D.S.; Choi, H.-J.; Koh, K. Enhancement of BSA Binding on Au Surfaces by calixbisazacrown Monolayer. Sensors 2007, 7, 2263-2272.View more citation formats
Chen H, Kim YS, Lee J, Yoon SJ, Lim DS, Choi H-J, Koh K. Enhancement of BSA Binding on Au Surfaces by calixbisazacrown Monolayer. Sensors. 2007; 7(10):2263-2272.Chicago/Turabian Style
Chen, Hongxia; Kim, Youn S.; Lee, Jaebeom; Yoon, Seok J.; Lim, Dong S.; Choi, Heung-Jin; Koh, Kwangnak. 2007. "Enhancement of BSA Binding on Au Surfaces by calixbisazacrown Monolayer." Sensors 7, no. 10: 2263-2272.
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