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Sensors 2007, 7(1), 52-66; doi:10.3390/s7010052
Article

A Non-invasive and Real-time Monitoring of the Regulation of Photosynthetic Metabolism Biosensor Based on Measurement of Delayed Fluorescence in Vivo

, *  and
Received: 12 November 2006; Accepted: 17 January 2007 / Published: 24 January 2007
(This article belongs to the Special Issue Physiological Sensing)
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Abstract: In this paper, a new principle biosensor for non-invasive monitoring of theregulation of photosynthetic metabolism based on quantitative measurement of delayedfluorescence (DF) is developed. The biosensor, which uses light-emitting diode lattice asexcitation light source and a compact Single Photon Counting Module to collect DF signal,is portable and can evaluate plant photosynthesis capacity in vivo. Compared with itsprimary version in our previous report, the biosensor can better control environmentalfactors. Moreover, the improved biosensor can automatically complete the measurements oflight and CO2 response curves of DF intensity. In the experimental study, the testing of theimproved biosensor has been made in soybean (Glycine max Zaoshu No. 18) seedlingstreated with NaHSO3 to induce changes in seedlings growth and photosynthetic metabolism.Contrast evaluations of seedlings photosynthesis were made from measurements of netphotosynthesis rate (Pn) based on consumption of CO2 in tested plants. Current testingresults have demonstrated that the improved biosensor can accurately determine theregulatory effects of NaHSO3 on photosynthetic metabolism. Therefore, the biosensorpresented here could be potential useful for real-time monitoring the regulatory effects ofplant growth regulators (PGRs) and other exogenous chemical factors on plant growth andphotosynthetic metabolism.
Keywords: biosensor; light-induced delayed fluorescence (DF); net photosynthesis rate (Pn); photosynthetic metabolism; plant growth regulators (PGRs). biosensor; light-induced delayed fluorescence (DF); net photosynthesis rate (Pn); photosynthetic metabolism; plant growth regulators (PGRs).
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Zhang, L.; Xing, D.; Wang, J. A Non-invasive and Real-time Monitoring of the Regulation of Photosynthetic Metabolism Biosensor Based on Measurement of Delayed Fluorescence in Vivo. Sensors 2007, 7, 52-66.

AMA Style

Zhang L, Xing D, Wang J. A Non-invasive and Real-time Monitoring of the Regulation of Photosynthetic Metabolism Biosensor Based on Measurement of Delayed Fluorescence in Vivo. Sensors. 2007; 7(1):52-66.

Chicago/Turabian Style

Zhang, Lingrui; Xing, Da; Wang, Junsheng. 2007. "A Non-invasive and Real-time Monitoring of the Regulation of Photosynthetic Metabolism Biosensor Based on Measurement of Delayed Fluorescence in Vivo." Sensors 7, no. 1: 52-66.


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