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Sensors 2005, 5(1), 70-84; doi:10.3390/s5010070
Article

Phytochelatin Modified Electrode Surface as a Sensitive Heavy- Metal Ion Biosensor

1,4, 1, 1, 1,4, 2, 3, 5, 6 and 1,*
Received: 6 September 2004; Accepted: 18 November 2004 / Published: 28 February 2005
(This article belongs to the Special Issue Sensors for Environmental Monitoring)
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Abstract: Electrochemical biosensors have superior properties over other existingmeasurement systems because they can provide rapid, simple and low-cost on-fielddetermination of many biological active species and a number of dangerous pollutants. Inour work, we suggested a new heavy metal biosensor based on interaction of heavy metalions (Cd2+ and Zn2+ ) with phytochelatin, which was adsorbed on the surface of the hangingmercury drop electrode, using adsorptive transfer stripping differential pulse voltammetry.In addition, we applied the suggested technique for the determination of heavy metals in abiological sample – human urine and platinum in a pharmaceutical drug. The detectionlimits (3 S/N) of Cd(II), Zn(II) and cis-platin were about 1.0, 13.3 and 1.9 pmole in 5 μl,respectively. On the basis of the obtained results, we propose that the suggested techniqueoffers simple, rapid, and low-cost detection of heavy metals in environmental, biologicaland medical samples.
Keywords: phytochelatin; adsorptive transfer stripping; differential pulse voltammetry; mercury; cadmium; zinc; heavy metal sensor; human urine; cis-platin. phytochelatin; adsorptive transfer stripping; differential pulse voltammetry; mercury; cadmium; zinc; heavy metal sensor; human urine; cis-platin.
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Adam, V.; Zehnalek, J.; Petrlova, J.; Potesil, D.; Sures, B.; Trnkova, L.; Jelen, F.; Vitecek, J.; Kizek, R. Phytochelatin Modified Electrode Surface as a Sensitive Heavy- Metal Ion Biosensor. Sensors 2005, 5, 70-84.

AMA Style

Adam V, Zehnalek J, Petrlova J, Potesil D, Sures B, Trnkova L, Jelen F, Vitecek J, Kizek R. Phytochelatin Modified Electrode Surface as a Sensitive Heavy- Metal Ion Biosensor. Sensors. 2005; 5(1):70-84.

Chicago/Turabian Style

Adam, Vojtech; Zehnalek, Josef; Petrlova, Jitka; Potesil, David; Sures, Bernd; Trnkova, Libuse; Jelen, Frantisek; Vitecek, Jan; Kizek, Rene. 2005. "Phytochelatin Modified Electrode Surface as a Sensitive Heavy- Metal Ion Biosensor." Sensors 5, no. 1: 70-84.



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