Open AccessThis article is
- freely available
Phytochelatin Modified Electrode Surface as a Sensitive Heavy- Metal Ion Biosensor
Department of Chemistry and Biochemistry and 6 Botany and Plant Physiology, Mendel University of Agriculture and Forestry, Zemedelska 1, 611 37 Brno, Czech Republic
Univ. Karlsruhe, Zool. Inst. Okol Parasitol 1, Karlsruhe, D-76128 Germany
Department of Theoretical and Physical Chemistry, Masaryk University, Faculty of Science, Kotlarska 2, 611 37 Brno, Czech Republic
Department of Analytical Chemistry, Masaryk University, Faculty of Science, Kotlarska 2, 611 37 Brno, Czech Republic
Laboratory of Biophysical Chemistry and Molecular Oncology, Institute of Biophysics, Academy of Sciences of the Czech Republic, Kralovopolska 135, Brno 612 65, Czech Republic
* Author to whom correspondence should be addressed.
Received: 6 September 2004; Accepted: 18 November 2004 / Published: 28 February 2005
Abstract: Electrochemical biosensors have superior properties over other existingmeasurement systems because they can provide rapid, simple and low-cost on-fielddetermination of many biological active species and a number of dangerous pollutants. Inour work, we suggested a new heavy metal biosensor based on interaction of heavy metalions (Cd2+ and Zn2+ ) with phytochelatin, which was adsorbed on the surface of the hangingmercury drop electrode, using adsorptive transfer stripping differential pulse voltammetry.In addition, we applied the suggested technique for the determination of heavy metals in abiological sample – human urine and platinum in a pharmaceutical drug. The detectionlimits (3 S/N) of Cd(II), Zn(II) and cis-platin were about 1.0, 13.3 and 1.9 pmole in 5 μl,respectively. On the basis of the obtained results, we propose that the suggested techniqueoffers simple, rapid, and low-cost detection of heavy metals in environmental, biologicaland medical samples.
Keywords: phytochelatin; adsorptive transfer stripping; differential pulse voltammetry; mercury; cadmium; zinc; heavy metal sensor; human urine; cis-platin.
Article StatisticsClick here to load and display the download statistics.
Notes: Multiple requests from the same IP address are counted as one view.
Cite This Article
MDPI and ACS Style
Adam, V.; Zehnalek, J.; Petrlova, J.; Potesil, D.; Sures, B.; Trnkova, L.; Jelen, F.; Vitecek, J.; Kizek, R. Phytochelatin Modified Electrode Surface as a Sensitive Heavy- Metal Ion Biosensor. Sensors 2005, 5, 70-84.
Adam V, Zehnalek J, Petrlova J, Potesil D, Sures B, Trnkova L, Jelen F, Vitecek J, Kizek R. Phytochelatin Modified Electrode Surface as a Sensitive Heavy- Metal Ion Biosensor. Sensors. 2005; 5(1):70-84.
Adam, Vojtech; Zehnalek, Josef; Petrlova, Jitka; Potesil, David; Sures, Bernd; Trnkova, Libuse; Jelen, Frantisek; Vitecek, Jan; Kizek, Rene. 2005. "Phytochelatin Modified Electrode Surface as a Sensitive Heavy- Metal Ion Biosensor." Sensors 5, no. 1: 70-84.