Phytochelatin Modified Electrode Surface as a Sensitive Heavy- Metal Ion Biosensor
AbstractElectrochemical biosensors have superior properties over other existingmeasurement systems because they can provide rapid, simple and low-cost on-fielddetermination of many biological active species and a number of dangerous pollutants. Inour work, we suggested a new heavy metal biosensor based on interaction of heavy metalions (Cd2+ and Zn2+ ) with phytochelatin, which was adsorbed on the surface of the hangingmercury drop electrode, using adsorptive transfer stripping differential pulse voltammetry.In addition, we applied the suggested technique for the determination of heavy metals in abiological sample – human urine and platinum in a pharmaceutical drug. The detectionlimits (3 S/N) of Cd(II), Zn(II) and cis-platin were about 1.0, 13.3 and 1.9 pmole in 5 μl,respectively. On the basis of the obtained results, we propose that the suggested techniqueoffers simple, rapid, and low-cost detection of heavy metals in environmental, biologicaland medical samples.
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Adam, V.; Zehnalek, J.; Petrlova, J.; Potesil, D.; Sures, B.; Trnkova, L.; Jelen, F.; Vitecek, J.; Kizek, R. Phytochelatin Modified Electrode Surface as a Sensitive Heavy- Metal Ion Biosensor. Sensors 2005, 5, 70-84.
Adam V, Zehnalek J, Petrlova J, Potesil D, Sures B, Trnkova L, Jelen F, Vitecek J, Kizek R. Phytochelatin Modified Electrode Surface as a Sensitive Heavy- Metal Ion Biosensor. Sensors. 2005; 5(1):70-84.Chicago/Turabian Style
Adam, Vojtech; Zehnalek, Josef; Petrlova, Jitka; Potesil, David; Sures, Bernd; Trnkova, Libuse; Jelen, Frantisek; Vitecek, Jan; Kizek, Rene. 2005. "Phytochelatin Modified Electrode Surface as a Sensitive Heavy- Metal Ion Biosensor." Sensors 5, no. 1: 70-84.