Sensors 2004, 4(6), 105-114; doi:10.3390/s40670105
Article

Electrical Characterization of a Thiol SAM on Gold as a First Step for the Fabrication of Immunosensors based on a Quartz Crystal Microbalance

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Received: 15 February 2004; Accepted: 18 August 2004 / Published: 30 August 2004
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: In order to develop a robust biosensor based on quartz crystal microbalance technique for antigen detection, a control of the steps of the surface functionalization has been performed by impedance spectroscopy. The gold electrode is functionalized with the self-assembled monolayer technique. The high insulating properties of the acidic thiol monolayer has been characterized with cyclic voltammetry and impedance spectroscopy. The modified surface is activated with N-hydroxysuccinimide(NHS) and 1-(3-(dimethylamino)propyl)-3-ethylcarbodimide hydrochloride(EDC) cross-linker for antibody coupling. The non-specific sites are blocked with bovin serum albumine molecules. Different concentrations of antigen can be detected with a good reversibility in real time with the quartz crystal microbalance.
Keywords: QCM; impedance spectroscopy; cyclic voltammetry; antibody; antigen
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MDPI and ACS Style

Tlili, A.; Abdelghani, A.; Hleli, S.; Maaref, M.A. Electrical Characterization of a Thiol SAM on Gold as a First Step for the Fabrication of Immunosensors based on a Quartz Crystal Microbalance. Sensors 2004, 4, 105-114.

AMA Style

Tlili A, Abdelghani A, Hleli S, Maaref MA. Electrical Characterization of a Thiol SAM on Gold as a First Step for the Fabrication of Immunosensors based on a Quartz Crystal Microbalance. Sensors. 2004; 4(6):105-114.

Chicago/Turabian Style

Tlili, Asma; Abdelghani, Adnane; Hleli, Salwa; Maaref, Mhamed A. 2004. "Electrical Characterization of a Thiol SAM on Gold as a First Step for the Fabrication of Immunosensors based on a Quartz Crystal Microbalance." Sensors 4, no. 6: 105-114.

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