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Sensors 2004, 4(6), 105-114; doi:10.3390/s40670105
Article

Electrical Characterization of a Thiol SAM on Gold as a First Step for the Fabrication of Immunosensors based on a Quartz Crystal Microbalance

, * ,  and
Unité de Recherche de Physique des Semiconducteurs et Capteurs, IPEST, La Marsa, 2070 Tunis, Tunisia
* Author to whom correspondence should be addressed.
Received: 15 February 2004 / Accepted: 18 August 2004 / Published: 30 August 2004
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Abstract

In order to develop a robust biosensor based on quartz crystal microbalance technique for antigen detection, a control of the steps of the surface functionalization has been performed by impedance spectroscopy. The gold electrode is functionalized with the self-assembled monolayer technique. The high insulating properties of the acidic thiol monolayer has been characterized with cyclic voltammetry and impedance spectroscopy. The modified surface is activated with N-hydroxysuccinimide(NHS) and 1-(3-(dimethylamino)propyl)-3-ethylcarbodimide hydrochloride(EDC) cross-linker for antibody coupling. The non-specific sites are blocked with bovin serum albumine molecules. Different concentrations of antigen can be detected with a good reversibility in real time with the quartz crystal microbalance.
Keywords: QCM; impedance spectroscopy; cyclic voltammetry; antibody; antigen QCM; impedance spectroscopy; cyclic voltammetry; antibody; antigen
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Tlili, A.; Abdelghani, A.; Hleli, S.; Maaref, M.A. Electrical Characterization of a Thiol SAM on Gold as a First Step for the Fabrication of Immunosensors based on a Quartz Crystal Microbalance. Sensors 2004, 4, 105-114.

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