Chemometric Classification of Herb – Orthosiphon stamineus According to Its Geographical Origin Using Virtual Chemical Sensor Based Upon Fast GC

Abstract: An analytical method using Electronic Nose (E-nose) instrument for analysis of volatile organic compound from Orthosiphon stamineus raw samples have been developed. This instrument is a new chemical sensor based on Fast Gas Chromatography and Surface Acoustics Wave (SAW) detector. Chromatographic fingerprint obtained from the headspace analysis of O. stamineus samples were used as a guideline for optimum selection of an array of sensor. Qualitative analysis was carried out based on the responses of each sensor array in order to distinguish the geographical origin of the cultivated sample. The results of the analysis showed variances of volatile chemical compound of the samples even though it is from the same species. However, similarities of main components from all five samples were observed. Usage of pattern recognition chemometric approaches such as Principal Component Analysis (PCA), Linear Discriminant Analysis (LDA) and Cluster Analysis (CA) for processing instrumental data provided good classification of O. stamineus samples according to its geographical origin.