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• Korbut, O
• Bučková, M
• Labuda, J
• Gründler, P
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• Korbut, O
• Bučková, M
• Labuda, J
• Gründler, P
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• Korbut, O
• Bučková, M
• Labuda, J
• Gründler, P

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Sensors 2003, 3(1), 1-10; doi:10.3390/s30100001

Article

Voltammetric Detection of Antioxidative Properties of Flavonoids Using Electrically Heated DNA Modified Carbon Paste Electrode

O. Korbut ¹ , M. Bučková ^2,* , J. Labuda ²  and P. Gründler ¹

¹ Department of Chemistry, Rostock University, 18051 Rostock, Germany ² Department of Analytical Chemistry, Slovak University of Technology, 81237 Bratislava, Slovak Republic

* Author to whom correspondence should be addressed.

Received: 7 November 2002 / Accepted: 15 November 2002 / Published: 24 January 2003

Download PDF Full-Text [215 KB, uploaded 1 October 2008 09:01 CEST]

Abstract: A simple electrochemical sensor consisting of electrically heated carbon paste electrode with the surface modified by dsDNA is used to characterize voltammetric behaviour and antioxidative activity of four selected flavonoids. Quercetin, rutin, catechin and epigallocatechin gallate accumulate within the DNA layer. A positive effect of the electrode temperature within the range of 20 to 38 ºC on the detection of a deep DNA degradation by a copper(II)/H₂O₂/ascorbic acid cleavage mixture as well as an antioxidative effect of flavonoids was evaluated.

Keywords: Flavonoids; Antioxidants; DNA biosensor; Carbon paste electrode; Heated electrode

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