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Sensors 2018, 18(8), 2605; https://doi.org/10.3390/s18082605

A “Turn-On” Fluorescence Copper Biosensor Based on DNA Cleavage-Dependent Graphene Oxide-dsDNA-CdTe Quantum Dots Complex

1
National Engineering Laboratory for Fiber Optic Sensing Technology, Wuhan University of Technology, Wuhan 430070, China
2
School of Information Engineering, Hubei University of Chinese Medicine, Wuhan 430065, China
*
Author to whom correspondence should be addressed.
Received: 9 July 2018 / Revised: 27 July 2018 / Accepted: 3 August 2018 / Published: 9 August 2018
(This article belongs to the Special Issue Graphene Based Sensors and Electronics)
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Abstract

A novel “turn-on” fluorescent copper biosensor is developed successfully based on the graphene oxide (GO)-dsDNA-CdTe quantum dots (QDs) complex via chemical crosslink method. The optical and structure properties of GO-dsDNA-CdTe QDs complex are studied by fluorescence (FL) spectra and transmission electron microscopy (TEM) in detail. It is demonstrated that the fluorescence quenching of CdTe QDs is a process of fluorescence resonance energy transfer (FRET) due to the essential surface and quenching properties of two-dimensional GO. Copper ions induce the catalytic reaction of DNA chain and irreversibly break at the cleavage site, which will cause the G-quadruplex formation, moreover further result in the CdTe QDs separated from GO and restored its fluorescence. Therefore, a significant recovery effect on the fluorescence of the GO-dsDNA-CdTe QDs complex is observed in the presence of copper ions. The fluorescence responses are concentration-dependent and can be well described by a linear equation. Compared with other metal ions, the sensor performs good selectivity for copper ions. View Full-Text
Keywords: copper ions detection; dsDNA-graphene oxide; CdTe quantum dots; fluorescence recovery copper ions detection; dsDNA-graphene oxide; CdTe quantum dots; fluorescence recovery
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Ding, L.; Xu, B.; Li, T.; Huang, J.; Bai, W. A “Turn-On” Fluorescence Copper Biosensor Based on DNA Cleavage-Dependent Graphene Oxide-dsDNA-CdTe Quantum Dots Complex. Sensors 2018, 18, 2605.

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