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Sensors 2018, 18(8), 2583; https://doi.org/10.3390/s18082583

Q3: A Compact Device for Quick, High Precision qPCR

1
STMicroelectronics S.r.l., I-20864 Agrate Brianza, Monza Brianza, Italy
2
CNR IMM—MDM, I-20864 Agrate Brianza, Monza Brianza, Italy
3
STMicroelectronics S.r.l., I-73100 Lecce, Italy
*
Author to whom correspondence should be addressed.
Received: 15 June 2018 / Revised: 27 July 2018 / Accepted: 2 August 2018 / Published: 7 August 2018
(This article belongs to the Special Issue Lab-on-a-Chip–From Point of Care to Precision Medicine)
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Abstract

An accurate and easy-to-use Q3 system for on-chip quantitative real-time Polymerase Chain Reaction (qPCR) is hereby demonstrated, and described in detail. The qPCR reactions take place inside a single-use Lab-on-a-Chip with multiple wells, each with 5 to 15 µL capacity. The same chip hosts a printed metal heater coupled with a calibrated sensor, for rapid and accurate temperature control inside the reaction mixture. The rest of the system is non-disposable and encased in a 7 × 14 × 8.5 (height) cm plastic shell weighing 300 g. Included in the non-disposable part is a fluorescence read-out system featuring up to four channels and a self-contained control and data storage system, interfacing with an external user-friendly software suite. Hereby, we illustrate the engineering details of the Q3 system and benchmark it with seamlessly ported testing protocols, showing that Q3 equals the performance of standard commercial systems. Overall, to the best of our knowledge, this is one of the most mature general-purpose systems for on-chip qPCR currently available. View Full-Text
Keywords: Lab-on-a-Chip; qPCR; real-time PCR; Q3; point-of-care tests; low-cost devices; miniaturization; on-chip diagnosis Lab-on-a-Chip; qPCR; real-time PCR; Q3; point-of-care tests; low-cost devices; miniaturization; on-chip diagnosis
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Cereda, M.; Cocci, A.; Cucchi, D.; Raia, L.; Pirola, D.; Bruno, L.; Ferrari, P.; Pavanati, V.; Calisti, G.; Ferrara, F.; Bramanti, A.P.; Bianchessi, M.A. Q3: A Compact Device for Quick, High Precision qPCR. Sensors 2018, 18, 2583.

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