Label-Free Quantification of Anti-TNF-α in Patients Treated with Adalimumab Using an Optical Biosensor
AbstractThis study describes the development of an immunosensory label-free quantification methodology based on surface plasmon resonance (SPR) and its applicability in measuring/evaluating therapeutic drug monitoring (TDM) of anti-TNF-α monoclonal antibody (adalimumab) in rheumatoid arthritis (RA) patients. The experimental parameters evaluated in this study were immobilising ligands by pre-concentration assays, sensor surface regeneration, ascertaining the method’s sensitivity and correlating the results from quantifying plasma samples by ELISA immunoassay. The results showed that TNF-α quantification values (in RU) were significantly different when comparing patients (~50–250 RU) to controls (~10–20 RU). Likewise, there was 0.97 correlation for patients and 0.91 for healthy volunteers using SPR and ELISA comparison methodologies. SPR immunosensory detection provided a precise, sensitive strategy, along with real-time determination, for quantifying adalimumab, having great potential for clinical routine regarding TDM. View Full-Text
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Bustos, R.H.; Zapata, C.; Esteban, E.; García, J.-C.; Jáuregui, E.; Jaimes, D. Label-Free Quantification of Anti-TNF-α in Patients Treated with Adalimumab Using an Optical Biosensor. Sensors 2018, 18, 691.
Bustos RH, Zapata C, Esteban E, García J-C, Jáuregui E, Jaimes D. Label-Free Quantification of Anti-TNF-α in Patients Treated with Adalimumab Using an Optical Biosensor. Sensors. 2018; 18(3):691.Chicago/Turabian Style
Bustos, Rosa H.; Zapata, Carlos; Esteban, Efraín; García, Julio-César; Jáuregui, Edwin; Jaimes, Diego. 2018. "Label-Free Quantification of Anti-TNF-α in Patients Treated with Adalimumab Using an Optical Biosensor." Sensors 18, no. 3: 691.
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