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Sensors 2018, 18(1), 49; https://doi.org/10.3390/s18010049

Amplified Detection of the Aptamer–Vanillin Complex with the Use of Bsm DNA Polymerase

Scientific-Manufacturing Complex Technological Centre, 1–7 Shokin Square, Zelenograd, 124498 Moscow, Russia
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Received: 30 November 2017 / Revised: 19 December 2017 / Accepted: 20 December 2017 / Published: 26 December 2017
(This article belongs to the Special Issue I3S 2017 Selected Papers)
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Abstract

The electrochemical detection of interactions between aptamers and low-molecular-weight targets often lacks sensitivity. Signal amplification improves the detection of the aptamer-analyte complex; Bsm DNA polymerase was used to amplify the signal from the interaction of vanillin and its aptamer named Van_74 on an ion-sensitive field-effect transistor (ISFET)-based biosensor. The aptamer was immobilized on the ISFET sensitive surface. A short DNA probe was hybridized with the aptamer and dissociated from it upon vanillin addition. A free probe interacted with a special DNA molecular beacon initiated the Bsm DNA polymerase reaction that was detected by ISFET. A buffer solution suitable for both aptamer action and Bsm DNA polymerase activity was determined. The ISFET was shown to detect the Bsm DNA polymerase reaction under the selected conditions. Vanillin at different concentrations (1 × 10−6–1 × 10−8 M) was detected using the biosensor with signal amplification. The developed detection system allowed for the determination of vanillin, starting at a 10−8 M concentration. Application of the Bsm DNA polymerase resulted in a 15.5 times lower LoD when compared to the biosensor without signal amplification (10.1007/s00604-017-2586-4). View Full-Text
Keywords: aptasensor; aptamer; signal amplification; Bsm DNA polymerase; ISFET; vanillin aptasensor; aptamer; signal amplification; Bsm DNA polymerase; ISFET; vanillin
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Andrianova, M.; Komarova, N.; Grudtsov, V.; Kuznetsov, E.; Kuznetsov, A. Amplified Detection of the Aptamer–Vanillin Complex with the Use of Bsm DNA Polymerase. Sensors 2018, 18, 49.

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