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Sensors 2017, 17(8), 1883; doi:10.3390/s17081883

A Microneedle Functionalized with Polyethyleneimine and Nanotubes for Highly Sensitive, Label-Free Quantification of DNA

School of Engineering and Computer Science, Washington State University, Vancouver, WA 98686, USA
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Received: 23 June 2017 / Revised: 2 August 2017 / Accepted: 14 August 2017 / Published: 16 August 2017
(This article belongs to the Special Issue Polymer-Based Sensors for Bioanalytes)
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Abstract

The accurate measure of DNA concentration is necessary for many DNA-based biological applications. However, the current methods are limited in terms of sensitivity, reproducibility, human error, and contamination. Here, we present a microneedle functionalized with polyethyleneimine (PEI) and single-walled carbon nanotubes (SWCNTs) for the highly sensitive quantification of DNA. The microneedle was fabricated using ultraviolet (UV) lithography and anisotropic etching, and then functionalized with PEI and SWCNTs through a dip coating process. The electrical characteristics of the microneedle change with the accumulation of DNA on the surface. Current-voltage measurements in deionized water were conducted to study these changes in the electrical properties of the sensor. The sensitivity test found the signal to be discernable from the noise level down to 100 attomolar (aM), demonstrating higher sensitivity than currently available UV fluorescence and UV absorbance based methods. A microneedle without any surface modification only had a 100 femtomolar (fM) sensitivity. All measurement results were consistent with fluorescence microscopy. View Full-Text
Keywords: amperometric sensors; carbon nanotubes; DNA; polyethyleneimine amperometric sensors; carbon nanotubes; DNA; polyethyleneimine
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Saadat-Moghaddam, D.; Kim, J.-H. A Microneedle Functionalized with Polyethyleneimine and Nanotubes for Highly Sensitive, Label-Free Quantification of DNA. Sensors 2017, 17, 1883.

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