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Sensors 2017, 17(7), 1495; doi:10.3390/s17071495

Digital Microfluidics for Nucleic Acid Amplification

i3N|CENIMAT, Departamento de Ciência dos Materiais, Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa, Campus de Caparica, 2829-516 Caparica, Portugal
UCIBIO, Departamento de Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa, Campus de Caparica, 2829-516 Caparica, Portugal
Authors to whom correspondence should be addressed.
Received: 27 May 2017 / Revised: 17 June 2017 / Accepted: 22 June 2017 / Published: 25 June 2017
(This article belongs to the Special Issue Microfluidic Sensors and Control Devices)
View Full-Text   |   Download PDF [3105 KB, uploaded 25 June 2017]   |  


Digital Microfluidics (DMF) has emerged as a disruptive methodology for the control and manipulation of low volume droplets. In DMF, each droplet acts as a single reactor, which allows for extensive multiparallelization of biological and chemical reactions at a much smaller scale. DMF devices open entirely new and promising pathways for multiplex analysis and reaction occurring in a miniaturized format, thus allowing for healthcare decentralization from major laboratories to point-of-care with accurate, robust and inexpensive molecular diagnostics. Here, we shall focus on DMF platforms specifically designed for nucleic acid amplification, which is key for molecular diagnostics of several diseases and conditions, from pathogen identification to cancer mutations detection. Particular attention will be given to the device architecture, materials and nucleic acid amplification applications in validated settings. View Full-Text
Keywords: Digital Microfluidics; nucleic acid amplification; point-of-care diagnostics Digital Microfluidics; nucleic acid amplification; point-of-care diagnostics

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Coelho, B.; Veigas, B.; Fortunato, E.; Martins, R.; Águas, H.; Igreja, R.; Baptista, P.V. Digital Microfluidics for Nucleic Acid Amplification. Sensors 2017, 17, 1495.

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