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Sensors 2017, 17(6), 1314; doi:10.3390/s17061314

Tyrosinase-Based Biosensors for Selective Dopamine Detection

Faculty of Medicine, Transilvania University of Brasov, 500019 Brasov, Romania
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Author to whom correspondence should be addressed.
Academic Editor: Alexander Star
Received: 3 May 2017 / Revised: 30 May 2017 / Accepted: 1 June 2017 / Published: 7 June 2017
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Abstract

A novel tyrosinase-based biosensor was developed for the detection of dopamine (DA). For increased selectivity, gold electrodes were previously modified with cobalt (II)-porphyrin (CoP) film with electrocatalytic activity, to act both as an electrochemical mediator and an enzyme support, upon which the enzyme tyrosinase (Tyr) was cross-linked. Differential pulse voltammetry was used for electrochemical detection and the reduction current of dopamine-quinone was measured as a function of dopamine concentration. Our experiments demonstrated that the presence of CoP improves the selectivity of the electrode towards dopamine in the presence of ascorbic acid (AA), with a linear trend of concentration dependence in the range of 2–30 µM. By optimizing the conditioning parameters, a separation of 130 mV between the peak potentials for ascorbic acid AA and DA was obtained, allowing the selective detection of DA. The biosensor had a sensitivity of 1.22 ± 0.02 µA·cm−2·µM−1 and a detection limit of 0.43 µM. Biosensor performances were tested in the presence of dopamine medication, with satisfactory results in terms of recovery (96%), and relative standard deviation values below 5%. These results confirmed the applicability of the biosensors in real samples such as human urine and blood serum. View Full-Text
Keywords: cobalt (II)-porphyrin; tyrosinase; electrochemical biosensors; dopamine cobalt (II)-porphyrin; tyrosinase; electrochemical biosensors; dopamine
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Florescu, M.; David, M. Tyrosinase-Based Biosensors for Selective Dopamine Detection. Sensors 2017, 17, 1314.

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