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Sensors 2017, 17(12), 2840; https://doi.org/10.3390/s17122840

Facile Determination of Sodium Ion and Osmolarity in Artificial Tears by Sequential DNAzymes

1,2
,
1,2
,
3,4,* and 1,2,4,5,*
1
Department of Life Science, Hanyang University, Seoul 04763, Korea
2
Research Institute for Natural Sciences, Hanyang University, Seoul 04763, Korea
3
Department of Bioengineering, Hanyang University, Seoul 04763, Korea
4
Institute of Nano Science and Technology, Hanyang University, Seoul 04763, Korea
5
Research Institute for Convergence of Basic Sciences, Hanyang University, Seoul 04763, Korea
*
Authors to whom correspondence should be addressed.
Received: 7 November 2017 / Revised: 29 November 2017 / Accepted: 5 December 2017 / Published: 7 December 2017
(This article belongs to the Special Issue Recent Advances in Nucleic Acid Sensors)
View Full-Text   |   Download PDF [1903 KB, uploaded 7 December 2017]   |  

Abstract

Despite high relevance of tear osmolarity and eye abnormality, numerous methods for detecting tear osmolarity rely upon expensive osmometers. We report a reliable method for simply determining sodium ion-based osmolarity in artificial tears using sequential DNAzymes. When sodium ion-specific DNAzyme and peroxidase-like DNAzyme were used as a sensing and detecting probe, respectively, the concentration of Na+ in artificial tears could be measured by absorbance or fluorescence intensity, which was highly correlated with osmolarity over the diagnostic range (R2 > 0.98). Our approach is useful for studying eye diseases in relation to osmolarity. View Full-Text
Keywords: DNAzyme; osmolarity; sodium ion; salinity; tear; dry eye DNAzyme; osmolarity; sodium ion; salinity; tear; dry eye
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Kim, E.H.; Lee, E.-S.; Lee, D.Y.; Kim, Y.-P. Facile Determination of Sodium Ion and Osmolarity in Artificial Tears by Sequential DNAzymes. Sensors 2017, 17, 2840.

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