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Sensors 2017, 17(11), 2616; https://doi.org/10.3390/s17112616

A Digital Microfluidics Platform for Loop-Mediated Isothermal Amplification Detection

1
CENIMAT|i3N, Departamento de Ciência dos Materiais, Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa, Campus de Caparica, 2829-516 Caparica, Portugal
2
UCIBIO, Departamento de Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa, Campus de Caparica, 2829-516 Caparica, Portugal
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Received: 4 October 2017 / Revised: 31 October 2017 / Accepted: 10 November 2017 / Published: 16 November 2017
(This article belongs to the Special Issue Microfluidic Sensors)
View Full-Text   |   Download PDF [3284 KB, uploaded 16 November 2017]   |  

Abstract

Digital microfluidics (DMF) arises as the next step in the fast-evolving field of operation platforms for molecular diagnostics. Moreover, isothermal schemes, such as loop-mediated isothermal amplification (LAMP), allow for further simplification of amplification protocols. Integrating DMF with LAMP will be at the core of a new generation of detection devices for effective molecular diagnostics at point-of-care (POC), providing simple, fast, and automated nucleic acid amplification with exceptional integration capabilities. Here, we demonstrate for the first time the role of coupling DMF and LAMP, in a dedicated device that allows straightforward mixing of LAMP reagents and target DNA, as well as optimum temperature control (reaction droplets undergo a temperature variation of just 0.3 °C, for 65 °C at the bottom plate). This device is produced using low-temperature and low-cost production processes, adaptable to disposable and flexible substrates. DMF-LAMP is performed with enhanced sensitivity without compromising reaction efficacy or losing reliability and efficiency, by LAMP-amplifying 0.5 ng/µL of target DNA in just 45 min. Moreover, on-chip LAMP was performed in 1.5 µL, a considerably lower volume than standard bench-top reactions. View Full-Text
Keywords: digital microfluidics; loop-mediated isothermal amplification; c-Myc; lab-on-a-chip; point-of-care diagnostics digital microfluidics; loop-mediated isothermal amplification; c-Myc; lab-on-a-chip; point-of-care diagnostics
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Coelho, B.J.; Veigas, B.; Águas, H.; Fortunato, E.; Martins, R.; Baptista, P.V.; Igreja, R. A Digital Microfluidics Platform for Loop-Mediated Isothermal Amplification Detection. Sensors 2017, 17, 2616.

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