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Sensors 2017, 17(1), 37; doi:10.3390/s17010037

Enhancement of Fluorescence-Based Sandwich Immunoassay Using Multilayered Microplates Modified with Plasma-Polymerized Films

School of Bioscience and Biotechnology, Tokyo University of Technology, 1404-1 Katakura, Hachioji, Tokyo 192-0982, Japan
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Academic Editors: Jong Seung Kim and Min Hee Lee
Received: 29 November 2016 / Revised: 21 December 2016 / Accepted: 22 December 2016 / Published: 25 December 2016
(This article belongs to the Special Issue Colorimetric and Fluorescent Sensor)
View Full-Text   |   Download PDF [2940 KB, uploaded 27 December 2016]   |  

Abstract

A functional modification of the surface of a 96-well microplate coupled with a thin layer deposition technique is demonstrated for enhanced fluorescence-based sandwich immunoassays. The plasma polymerization technique enabling the deposition of organic thin films was employed for the modification of the well surface of a microplate. A silver layer and a plasma-polymerized film were consecutively deposited on the microplate as a metal mirror and the optical interference layer, respectively. When Cy3-labeled antibody was applied to the wells of the resulting multilayered microplate without any immobilization step, greatly enhanced fluorescence was observed compared with that obtained with the unmodified one. The same effect could be also exhibited for an immunoassay targeting antigen directly adsorbed on the multilayered microplate. Furthermore, a sandwich immunoassay for the detection of interleukin 2 (IL-2) was performed with the multilayered microplates, resulting in specific and 88-fold–enhanced fluorescence detection. View Full-Text
Keywords: microplate; plasma polymerization; sandwich-immunoassay; fluorescence enhancement; 1. Introduction microplate; plasma polymerization; sandwich-immunoassay; fluorescence enhancement; 1. Introduction
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Yano, K.; Iwasaki, A. Enhancement of Fluorescence-Based Sandwich Immunoassay Using Multilayered Microplates Modified with Plasma-Polymerized Films. Sensors 2017, 17, 37.

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