Next Article in Journal
Dynamic Measurement for the Diameter of A Train Wheel Based on Structured-Light Vision
Next Article in Special Issue
Enzyme Biosensors for Biomedical Applications: Strategies for Safeguarding Analytical Performances in Biological Fluids
Previous Article in Journal
Stability Analysis of Multi-Sensor Kalman Filtering over Lossy Networks
Previous Article in Special Issue
One-Pot Hydrothermal Synthesis of Magnetite Prussian Blue Nano-Composites and Their Application to Fabricate Glucose Biosensor
Article Menu

Export Article

Open AccessArticle
Sensors 2016, 16(4), 563; doi:10.3390/s16040563

A Stimuli-Responsive Biosensor of Glucose on Layer-by-Layer Films Assembled through Specific Lectin-Glycoenzyme Recognition

1
Department of Pharmacy, Ningxia Medical University, Yinchuan 750004, China
2
State Key Laboratory Cultivation Base of Natural Gas Conversion, Ningxia University, Yinchuan 750021, China
3
School of Chemistry and Chemical Engineering, Ningxia University, Yinchuan 750021, China
*
Authors to whom correspondence should be addressed.
Academic Editor: Yu Lei
Received: 8 March 2016 / Revised: 13 April 2016 / Accepted: 18 April 2016 / Published: 20 April 2016
(This article belongs to the Special Issue Microbial and Enzymatic Biosensors)
View Full-Text   |   Download PDF [2164 KB, uploaded 20 April 2016]   |  

Abstract

The research on intelligent bioelectrocatalysis based on stimuli-responsive materials or interfaces is of great significance for biosensors and other bioelectronic devices. In the present work, lectin protein concanavalin A (Con A) and glycoenzyme glucose oxidase (GOD) were assembled into {Con A/GOD}n layer-by-layer (LbL) films by taking advantage of the biospecific lectin-glycoenzyme affinity between them. These film electrodes possess stimuli-responsive properties toward electroactive probes such as ferrocenedicarboxylic acid (Fc(COOH)2) by modulating the surrounding pH. The CV peak currents of Fc(COOH)2 were quite large at pH 4.0 but significantly suppressed at pH 8.0, demonstrating reversible stimuli-responsive on-off behavior. The mechanism of stimuli-responsive property of the films was explored by comparative experiments and attributed to the different electrostatic interaction between the films and the probes at different pH. This stimuli-responsive films could be used to realize active/inactive electrocatalytic oxidation of glucose by GOD in the films and mediated by Fc(COOH)2 in solution, which may establish a foundation for fabricating novel stimuli-responsive electrochemical biosensors based on bioelectrocatalysis with immobilized enzymes. View Full-Text
Keywords: stimuli-responsive biosensor; lectin-sugar specific interaction; layer-by-layer assembly; concanavalin A; enzyme immobilization; ferrocenedicarboxylic acid stimuli-responsive biosensor; lectin-sugar specific interaction; layer-by-layer assembly; concanavalin A; enzyme immobilization; ferrocenedicarboxylic acid
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Yao, H.; Gan, Q.; Peng, J.; Huang, S.; Zhu, M.; Shi, K. A Stimuli-Responsive Biosensor of Glucose on Layer-by-Layer Films Assembled through Specific Lectin-Glycoenzyme Recognition. Sensors 2016, 16, 563.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top