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Sensors 2016, 16(3), 374; doi:10.3390/s16030374

A Novel Conductive Poly(3,4-ethylenedioxythiophene)-BSA Film for the Construction of a Durable HRP Biosensor Modified with NanoAu Particles

1
Department of Chemical and Biochemical Engineering, Xiamen University, No. 422, Siming South Road, Xiamen 361005, Fujian, China
2
Department of Chemical and Materials Engineering, Tunghai University, No.1727, Sec.4, Taiwan Boulevard, Xitun District, Taichung 40704, Taiwan
*
Author to whom correspondence should be addressed.
Academic Editor: Roberto Pilloton
Received: 5 January 2016 / Revised: 4 March 2016 / Accepted: 7 March 2016 / Published: 15 March 2016
(This article belongs to the Special Issue Amperometric Biosensors)
View Full-Text   |   Download PDF [3596 KB, uploaded 15 March 2016]   |  

Abstract

In this study, we have investigated the contribution of bovine serum albumin (BSA) to the durability of the electrochemically synthesized poly(3,4-ethylenedioxythiophene) (PEDOT) film on a platinum (Pt) electrode. The electrode was capable to effectively adsorb the nano Au particles (AuNPs) to form a uniform layout, which was then able to immobilize the horseradish peroxidase (HRP) to construct a functional HRP/AuNPs/PEDOT(BSA)/Pt biosensor. Cyclic voltammetry was employed to evaluate the performance of the biosensor through the measurement of hydrogen peroxide. Our results revealed a satisfied linear correlation between the cathodic current and the concentration of H2O2. Furthermore, the addition of oxidized form of nicotinamide adenine dinucleotide, or NAD+, as the electron transfer mediator in the detection solution could dramatically enhance the sensitivity of detection by about 35.5%. The main advantages of the current biosensor are its durability, sensitivity, reliability, and biocompatibility. View Full-Text
Keywords: bovine serum albumin; poly(3,4-ethylenedioxythiophene); nano Au particle; HRP biosensor; electron transfer mediator bovine serum albumin; poly(3,4-ethylenedioxythiophene); nano Au particle; HRP biosensor; electron transfer mediator
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Xu, F.; Ren, S.; Gu, Y. A Novel Conductive Poly(3,4-ethylenedioxythiophene)-BSA Film for the Construction of a Durable HRP Biosensor Modified with NanoAu Particles. Sensors 2016, 16, 374.

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