Investigating Effects of Proteasome Inhibitor on Multiple Myeloma Cells Using Confocal Raman Microscopy
AbstractDue to its label-free and non-destructive nature, applications of Raman spectroscopic imaging in monitoring therapeutic responses at the cellular level are growing. We have recently developed a high-speed confocal Raman microscopy system to image living biological specimens with high spatial resolution and sensitivity. In the present study, we have applied this system to monitor the effects of Bortezomib, a proteasome inhibitor drug, on multiple myeloma cells. Cluster imaging followed by spectral profiling suggest major differences in the nuclear and cytoplasmic contents of cells due to drug treatment that can be monitored with Raman spectroscopy. Spectra were also acquired from group of cells and feasibility of discrimination among treated and untreated cells using principal component analysis (PCA) was accessed. Findings support the feasibility of Raman technologies as an alternate, novel method for monitoring live cell dynamics with minimal external perturbation. View Full-Text
Scifeed alert for new publicationsNever miss any articles matching your research from any publisher
- Get alerts for new papers matching your research
- Find out the new papers from selected authors
- Updated daily for 49'000+ journals and 6000+ publishers
- Define your Scifeed now
Kang, J.W.; Singh, S.P.; Nguyen, F.T.; Lue, N.; Sung, Y.; So, P.T.C.; Dasari, R.R. Investigating Effects of Proteasome Inhibitor on Multiple Myeloma Cells Using Confocal Raman Microscopy. Sensors 2016, 16, 2133.
Kang JW, Singh SP, Nguyen FT, Lue N, Sung Y, So PTC, Dasari RR. Investigating Effects of Proteasome Inhibitor on Multiple Myeloma Cells Using Confocal Raman Microscopy. Sensors. 2016; 16(12):2133.Chicago/Turabian Style
Kang, Jeon W.; Singh, Surya P.; Nguyen, Freddy T.; Lue, Niyom; Sung, Yongjin; So, Peter T.C.; Dasari, Ramachandra R. 2016. "Investigating Effects of Proteasome Inhibitor on Multiple Myeloma Cells Using Confocal Raman Microscopy." Sensors 16, no. 12: 2133.
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.