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Sensors 2016, 16(12), 2133; doi:10.3390/s16122133

Investigating Effects of Proteasome Inhibitor on Multiple Myeloma Cells Using Confocal Raman Microscopy

1
Laser Biomedical Research Center, G. R. Harrison Spectroscopy Laboratory, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
2
Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
3
Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
4
Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
*
Author to whom correspondence should be addressed.
Academic Editors: Alexandre François, Al Meldrum and Nicolas Riesen
Received: 19 November 2016 / Revised: 5 December 2016 / Accepted: 12 December 2016 / Published: 14 December 2016
(This article belongs to the Special Issue Label-Free Optical Biosensors)
View Full-Text   |   Download PDF [2019 KB, uploaded 14 December 2016]   |  

Abstract

Due to its label-free and non-destructive nature, applications of Raman spectroscopic imaging in monitoring therapeutic responses at the cellular level are growing. We have recently developed a high-speed confocal Raman microscopy system to image living biological specimens with high spatial resolution and sensitivity. In the present study, we have applied this system to monitor the effects of Bortezomib, a proteasome inhibitor drug, on multiple myeloma cells. Cluster imaging followed by spectral profiling suggest major differences in the nuclear and cytoplasmic contents of cells due to drug treatment that can be monitored with Raman spectroscopy. Spectra were also acquired from group of cells and feasibility of discrimination among treated and untreated cells using principal component analysis (PCA) was accessed. Findings support the feasibility of Raman technologies as an alternate, novel method for monitoring live cell dynamics with minimal external perturbation. View Full-Text
Keywords: Raman spectroscopy; Raman microscopy; therapeutic response monitoring; cell imaging Raman spectroscopy; Raman microscopy; therapeutic response monitoring; cell imaging
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MDPI and ACS Style

Kang, J.W.; Singh, S.P.; Nguyen, F.T.; Lue, N.; Sung, Y.; So, P.T.C.; Dasari, R.R. Investigating Effects of Proteasome Inhibitor on Multiple Myeloma Cells Using Confocal Raman Microscopy. Sensors 2016, 16, 2133.

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