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Sensors 2015, 15(8), 18565-18578; doi:10.3390/s150818565

An Impedance Aptasensor with Microfluidic Chips for Specific Detection of H5N1 Avian Influenza Virus

1
Cell and Molecular Biology Program, University of Arkansas, Fayetteville, AR 72701, USA
2
Department of Biological and Agricultural Engineering, University of Arkansas, Fayetteville, AR 72701, USA
3
Department of Poultry Science, University of Arkansas, Fayetteville, AR 72701, USA
4
Department of Mechanical Engineering, University of Arkansas, Fayetteville, AR 72701, USA
5
Animal Diagnostic Laboratory, Pennsylvania State University, University Park, PA 16802, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Kagan Kerman
Received: 4 June 2015 / Revised: 17 July 2015 / Accepted: 21 July 2015 / Published: 29 July 2015
View Full-Text   |   Download PDF [1078 KB, uploaded 29 July 2015]   |  

Abstract

In this research a DNA aptamer, which was selected through SELEX (systematic evolution of ligands by exponential enrichment) to be specific against the H5N1 subtype of the avian influenza virus (AIV), was used as an alternative reagent to monoclonal antibodies in an impedance biosensor utilizing a microfluidics flow cell and an interdigitated microelectrode for the specific detection of H5N1 AIV. The gold surface of the interdigitated microelectrode embedded in a microfluidics flow cell was modified using streptavidin. The biotinylated aptamer against H5N1 was then immobilized on the electrode surface using biotin–streptavidin binding. The target virus was captured on the microelectrode surface, causing an increase in impedance magnitude. The aptasensor had a detection time of 30 min with a detection limit of 0.0128 hemagglutinin units (HAU). Scanning electron microscopy confirmed the binding of the target virus onto the electrode surface. The DNA aptamer was specific to H5N1 and had no cross-reaction to other subtypes of AIV (e.g., H1N1, H2N2, H7N2). The newly developed aptasensor offers a portable, rapid, low-cost alternative to current methods with the same sensitivity and specificity. View Full-Text
Keywords: impedance aptasensor; avian influenza virus; aptamer; microfluidic chip; virus detection impedance aptasensor; avian influenza virus; aptamer; microfluidic chip; virus detection
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Lum, J.; Wang, R.; Hargis, B.; Tung, S.; Bottje, W.; Lu, H.; Li, Y. An Impedance Aptasensor with Microfluidic Chips for Specific Detection of H5N1 Avian Influenza Virus. Sensors 2015, 15, 18565-18578.

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