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Sensors 2015, 15(5), 11417-11430; doi:10.3390/s150511417

Fluorescence Characteristics and Lifetime Images of Photosensitizers of Talaporfin Sodium and Sodium Pheophorbide a in Normal and Cancer Cells

1
Research Institute for Electronic Science (RIES), Hokkaido University, Sapporo 001-0020, Japan
2
Graduate School of Photonic Science, Chitose Institute for Science and Technology, Chitose 066-8655, Japan
3
Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba-ku, Sendai 980-8578, Japan
Present address: Department of Applied Chemistry and Institute of Molecular Science, National Chiao Tung University, 1001 Ta-Hsueh Road, Hsinchu 30010, Taiwan
*
Authors to whom correspondence should be addressed.
Academic Editor: Magnus Willander
Received: 19 March 2015 / Revised: 1 May 2015 / Accepted: 11 May 2015 / Published: 18 May 2015
(This article belongs to the Special Issue Intracellular Sensing)
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Abstract

Fluorescence spectra and fluorescence lifetime images of talaporfin sodium and sodium-pheophorbide a, which can be regarded as photosensitizers for photodynamic therapy, were measured in normal and cancer cells. The reduction of the fluorescence intensity by photoirradiation was observed for both photosensitizers in both cells, but the quenching rate was much faster in cancer cells than in normal cells. These results are explained in terms of the excessive generation of reactive oxygen species via photoexcitation of these photosensitizers in cancer cells. The fluorescence lifetimes of both photosensitizers in cancer cells are different from those in normal cells, which originates from the different intracellular environments around the photosensitizers between normal and cancer cells. View Full-Text
Keywords: sodium pheophorbide a; talaporfin sodium; photodynamic therapy; reactive oxygen species; cancer cells; fluorescence spectrum; fluorescence lifetime sodium pheophorbide a; talaporfin sodium; photodynamic therapy; reactive oxygen species; cancer cells; fluorescence spectrum; fluorescence lifetime
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Awasthi, K.; Yamamoto, K.; Furuya, K.; Nakabayashi, T.; Li, L.; Ohta, N. Fluorescence Characteristics and Lifetime Images of Photosensitizers of Talaporfin Sodium and Sodium Pheophorbide a in Normal and Cancer Cells. Sensors 2015, 15, 11417-11430.

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