Next Article in Journal
Development of an Ultrasonic Airflow Measurement Device for Ducted Air
Next Article in Special Issue
Diamond Nanowires: A Novel Platform for Electrochemistry and Matrix-Free Mass Spectrometry
Previous Article in Journal
Classifying Step and Spin Turns Using Wireless Gyroscopes and Implications for Fall Risk Assessments
Article Menu

Export Article

Open AccessArticle
Sensors 2015, 15(5), 10686-10704; doi:10.3390/s150510686

A Label-Free Impedimetric DNA Sensor Based on a Nanoporous SnO2 Film: Fabrication and Detection Performance

1
Université Grenoble-Alpes, CNRS, Laboratoire des Matériaux et du Génie Physique (LMGP), MINATEC, 3 parvis Louis Néel, 38016 Grenoble Cedex 1, France
2
School of Materials Science and Engineering, Hanoi University of Science and Technology, 1 Dai Co Viet Street, 10000 Hanoi, Vietnam
3
Laboratoire d'Electrochimie et de Physico-chimie des Matériaux et des Interfaces (LEPMI), 1130 rue de la Piscine BP75, 38402 Saint Martin d'Hères Cedex, France
*
Author to whom correspondence should be addressed.
Academic Editor: Stephen Holler
Received: 12 March 2015 / Revised: 27 April 2015 / Accepted: 4 May 2015 / Published: 6 May 2015
(This article belongs to the Special Issue Label-Free Sensing)
View Full-Text   |   Download PDF [460 KB, uploaded 7 May 2015]   |  

Abstract

Nanoporous SnO2 thin films were elaborated to serve as sensing electrodes for label-free DNA detection using electrochemical impedance spectroscopy (EIS). Films were deposited by an electrodeposition process (EDP). Then the non-Faradic EIS behaviour was thoroughly investigated during some different steps of functionalization up to DNA hybridization. The results have shown a systematic decrease of the impedance upon DNA hybridization. The impedance decrease is attributed to an enhanced penetration of ionic species within the film volume. Besides, the comparison of impedance variations upon DNA hybridization between the liquid and vapour phase processes for organosilane (APTES) grafting on the nanoporous SnO2 films showed that vapour-phase method is more efficient. This is due to the fact that the vapour is more effective than the solution in penetrating the nanopores of the films. As a result, the DNA sensors built from vapour-treated silane layer exhibit a higher sensitivity than those produced from liquid-treated silane, in the range of tested target DNA concentration going to 10 nM. Finally, the impedance and fluorescence response signals strongly depend on the types of target DNA molecules, demonstrating a high selectivity of the process on nanoporous SnO2 films. View Full-Text
Keywords: biosensor; DNA; label free detection, fluorescence; impedance spectroscopy; nanoporous SnO2 films biosensor; DNA; label free detection, fluorescence; impedance spectroscopy; nanoporous SnO2 films
Figures

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Le, M.H.; Jimenez, C.; Chainet, E.; Stambouli, V. A Label-Free Impedimetric DNA Sensor Based on a Nanoporous SnO2 Film: Fabrication and Detection Performance. Sensors 2015, 15, 10686-10704.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top