Next Article in Journal
An Overview of Distributed Microgrid State Estimation and Control for Smart Grids
Next Article in Special Issue
Sensitive, Fast, and Specific Immunoassays for Methyltestosterone Detection
Previous Article in Journal
An Accurate Link Correlation Estimator for Improving Wireless Protocol Performance
Previous Article in Special Issue
Nano-Magnetic Immunosensor Based on Staphylococcus Protein A and the Amplification Effect of HRP-Conjugated Phage Antibody
Article Menu

Export Article

Open AccessArticle
Sensors 2015, 15(2), 4291-4301; doi:10.3390/s150204291

Development of an Immunochromatographic Strip for Rapid Detection of Pantoea stewartii subsp. stewartii

1
Huaian Entry-Exit Inspection and Quarantine Bureau, Huaian 223001, China
2
State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China
*
Author to whom correspondence should be addressed.
Received: 12 December 2014 / Revised: 4 January 2015 / Accepted: 3 February 2015 / Published: 12 February 2015
(This article belongs to the Special Issue Immunosensors 2014)
View Full-Text   |   Download PDF [413 KB, uploaded 13 February 2015]   |  

Abstract

A rapid, simple, sensitive, and specific immunochromatographic test strip was developed for the detection of Pantoea stewartii subsp. stewartii (Pss) in corn seed which was soaked overnight and then centrifuged for precipitate re-dissolved as samples. A pair of sensitive monoclonal antibodies for the immunochromatographic test strip was generated by mice immunization and cell fusion. Under optimized conditions, the lower detection limit of the strips for Pss was 1 × 105 cfu/mL both in 0.01 M phosphate buffer solution and corn seed samples, with no cross-reactivity with other common plant pathogens. The developed strip is useful and rapid for the detection of Pss in corn seed samples. View Full-Text
Keywords: Pantoea stewartii subsp. stewartii (Pss); immunochromatographic test strip; monoclonal antibody Pantoea stewartii subsp. stewartii (Pss); immunochromatographic test strip; monoclonal antibody
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary materials

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Feng, M.; Kong, D.; Wang, W.; Liu, L.; Song, S.; Xu, C. Development of an Immunochromatographic Strip for Rapid Detection of Pantoea stewartii subsp. stewartii. Sensors 2015, 15, 4291-4301.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top