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Sensors 2015, 15(1), 1047-1059; doi:10.3390/s150101047

Accelerated Detection of Viral Particles by Combining AC Electric Field Effects and Micro-Raman Spectroscopy

Department of Chemical Engineering Queen's University, Kingston, ON K7L 3N6, Canada
Present address: FP Innovations, 2665 East Mall, Vancouver, BC V6T1Z4, Canada
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Author to whom correspondence should be addressed.
Received: 4 September 2014 / Accepted: 30 December 2014 / Published: 8 January 2015
(This article belongs to the Special Issue Advances in Optical Biosensors)
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Abstract

A detection method that combines electric field-assisted virus capture on antibody-decorated surfaces with the “fingerprinting” capabilities of micro-Raman spectroscopy is demonstrated for the case of M13 virus in water. The proof-of-principle surface mapping of model bioparticles (protein coated polystyrene spheres) captured by an AC electric field between planar microelectrodes is presented with a methodology for analyzing the resulting spectra by comparing relative peak intensities. The same principle is applied to dielectrophoretically captured M13 phage particles whose presence is indirectly confirmed with micro-Raman spectroscopy using NeutrAvidin-Cy3 as a labeling molecule. It is concluded that the combination of electrokinetically driven virus sampling and micro-Raman based signal transduction provides a promising approach for time-efficient and in situ detection of viruses. View Full-Text
Keywords: micro-Raman spectroscopy; AC electrokinetics; dielectrophoresis; microelectrodes; optical biosensors; M13 phage micro-Raman spectroscopy; AC electrokinetics; dielectrophoresis; microelectrodes; optical biosensors; M13 phage
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Tomkins, M.R.; Liao, D.S.; Docoslis, A. Accelerated Detection of Viral Particles by Combining AC Electric Field Effects and Micro-Raman Spectroscopy. Sensors 2015, 15, 1047-1059.

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