Abstract: The following paper examines a time-efficient method for detecting biological warfare agents (BWAs). The method is based on a system of a Love-wave immunosensor combined with a microfluidic chip which detects BWA samples in a dynamic mode. In this way a continuous flow-through of the sample is created, promoting the reaction between antigen and antibody and allowing a fast detection of the BWAs. In order to prove this method, static and dynamic modes have been simulated and different concentrations of BWA simulants have been tested with two immunoreactions: phage M13 has been detected using the mouse monoclonal antibody anti-M13 (AM13), and the rabbit immunoglobulin (Rabbit IgG) has been detected using the polyclonal antibody goat anti-rabbit (GAR). Finally, different concentrations of each BWA simulants have been detected with a fast response time and a desirable level of discrimination among them has been achieved.
Keywords: biological warfare agent; BWA; love-wave; sensor acoustic wave; SAW; biosensor; immunosensor; microfluidics; bacteriophage
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Matatagui, D.; Fontecha, J.L.; Fernández, M.J.; Gràcia, I.; Cané, C.; Santos, J.P.; Horrillo, M.C. Love-Wave Sensors Combined with Microfluidics for Fast Detection of Biological Warfare Agents. Sensors 2014, 14, 12658-12669.
Matatagui D, Fontecha JL, Fernández MJ, Gràcia I, Cané C, Santos JP, Horrillo MC. Love-Wave Sensors Combined with Microfluidics for Fast Detection of Biological Warfare Agents. Sensors. 2014; 14(7):12658-12669.
Matatagui, Daniel; Fontecha, José L.; Fernández, María J.; Gràcia, Isabel; Cané, Carles; Santos, José P.; Horrillo, María C. 2014. "Love-Wave Sensors Combined with Microfluidics for Fast Detection of Biological Warfare Agents." Sensors 14, no. 7: 12658-12669.