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Sensors 2014, 14(7), 12658-12669; doi:10.3390/s140712658
Article

Love-Wave Sensors Combined with Microfluidics for Fast Detection of Biological Warfare Agents

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1,*
1 Instituto de Tecnologías Físicas y de la Información (ITEFI), CSIC, Serrano 144, Madrid 28006, Spain 2 Centro de Ciencias Aplicadas y Desarrollo Tecnológico (CCADET), Universidad Nacional Autónoma de México (UNAM), 04510 Distrito Federal, Mexico 3 Instituto de Microelectrónica de Barcelona, CSIC, Campus UAB, Bellaterra 08193, Spain
* Author to whom correspondence should be addressed.
Received: 1 May 2014 / Revised: 2 July 2014 / Accepted: 3 July 2014 / Published: 15 July 2014
(This article belongs to the Special Issue Chemo- and Biosensors for Security and Defense)
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Abstract

The following paper examines a time-efficient method for detecting biological warfare agents (BWAs). The method is based on a system of a Love-wave immunosensor combined with a microfluidic chip which detects BWA samples in a dynamic mode. In this way a continuous flow-through of the sample is created, promoting the reaction between antigen and antibody and allowing a fast detection of the BWAs. In order to prove this method, static and dynamic modes have been simulated and different concentrations of BWA simulants have been tested with two immunoreactions: phage M13 has been detected using the mouse monoclonal antibody anti-M13 (AM13), and the rabbit immunoglobulin (Rabbit IgG) has been detected using the polyclonal antibody goat anti-rabbit (GAR). Finally, different concentrations of each BWA simulants have been detected with a fast response time and a desirable level of discrimination among them has been achieved.
Keywords: biological warfare agent; BWA; love-wave; sensor acoustic wave; SAW; biosensor; immunosensor; microfluidics; bacteriophage biological warfare agent; BWA; love-wave; sensor acoustic wave; SAW; biosensor; immunosensor; microfluidics; bacteriophage
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Matatagui, D.; Fontecha, J.L.; Fernández, M.J.; Gràcia, I.; Cané, C.; Santos, J.P.; Horrillo, M.C. Love-Wave Sensors Combined with Microfluidics for Fast Detection of Biological Warfare Agents. Sensors 2014, 14, 12658-12669.

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