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Sensors 2014, 14(7), 12658-12669; doi:10.3390/s140712658
Article

Love-Wave Sensors Combined with Microfluidics for Fast Detection of Biological Warfare Agents

1,2
, 1
, 1
, 3
, 3
, 1
 and 1,*
1 Instituto de Tecnologías Físicas y de la Información (ITEFI), CSIC, Serrano 144, Madrid 28006, Spain 2 Centro de Ciencias Aplicadas y Desarrollo Tecnológico (CCADET), Universidad Nacional Autónoma de México (UNAM), 04510 Distrito Federal, Mexico 3 Instituto de Microelectrónica de Barcelona, CSIC, Campus UAB, Bellaterra 08193, Spain
* Author to whom correspondence should be addressed.
Received: 1 May 2014 / Revised: 2 July 2014 / Accepted: 3 July 2014 / Published: 15 July 2014
(This article belongs to the Special Issue Chemo- and Biosensors for Security and Defense)
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Abstract

The following paper examines a time-efficient method for detecting biological warfare agents (BWAs). The method is based on a system of a Love-wave immunosensor combined with a microfluidic chip which detects BWA samples in a dynamic mode. In this way a continuous flow-through of the sample is created, promoting the reaction between antigen and antibody and allowing a fast detection of the BWAs. In order to prove this method, static and dynamic modes have been simulated and different concentrations of BWA simulants have been tested with two immunoreactions: phage M13 has been detected using the mouse monoclonal antibody anti-M13 (AM13), and the rabbit immunoglobulin (Rabbit IgG) has been detected using the polyclonal antibody goat anti-rabbit (GAR). Finally, different concentrations of each BWA simulants have been detected with a fast response time and a desirable level of discrimination among them has been achieved.
Keywords: biological warfare agent; BWA; love-wave; sensor acoustic wave; SAW; biosensor; immunosensor; microfluidics; bacteriophage biological warfare agent; BWA; love-wave; sensor acoustic wave; SAW; biosensor; immunosensor; microfluidics; bacteriophage
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Matatagui, D.; Fontecha, J.L.; Fernández, M.J.; Gràcia, I.; Cané, C.; Santos, J.P.; Horrillo, M.C. Love-Wave Sensors Combined with Microfluidics for Fast Detection of Biological Warfare Agents. Sensors 2014, 14, 12658-12669.

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