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Sensors 2014, 14(5), 8718-8727; doi:10.3390/s140508718
Article

New Trends in Quantitative Assessment of the Corneal Barrier Function

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Received: 30 December 2013; in revised form: 26 April 2014 / Accepted: 5 May 2014 / Published: 16 May 2014
(This article belongs to the Special Issue State-of-the-Art Sensors Technology in Spain 2013)
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Abstract: The cornea is a very particular tissue due to its transparency and its barrier function as it has to resist against the daily insults of the external environment. In addition, maintenance of this barrier function is of crucial importance to ensure a correct corneal homeostasis. Here, the corneal epithelial permeability has been assessed in vivo by means of non-invasive tetrapolar impedance measurements, taking advantage of the huge impact of the ion fluxes in the passive electrical properties of living tissues. This has been possible by using a flexible sensor based in SU-8 photoresist. In this work, a further analysis focused on the validation of the presented sensor is performed by monitoring the healing process of corneas that were previously wounded. The obtained impedance measurements have been compared with the damaged area observed in corneal fluorescein staining images. The successful results confirm the feasibility of this novel method, as it represents a more sensitive in vivo and non-invasive test to assess low alterations of the epithelial permeability. Then, it could be used as an excellent complement to the fluorescein staining image evaluation.
Keywords: electrical impedance spectroscopy; corneal epithelium; permeability; barrier function; translayer electrical resistance; non-invasive; wound healing process electrical impedance spectroscopy; corneal epithelium; permeability; barrier function; translayer electrical resistance; non-invasive; wound healing process
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Guimerà, A.; Illa, X.; Traver, E.; Herrero, C.; Maldonado, M.J.; Villa, R. New Trends in Quantitative Assessment of the Corneal Barrier Function. Sensors 2014, 14, 8718-8727.

AMA Style

Guimerà A, Illa X, Traver E, Herrero C, Maldonado MJ, Villa R. New Trends in Quantitative Assessment of the Corneal Barrier Function. Sensors. 2014; 14(5):8718-8727.

Chicago/Turabian Style

Guimerà, Anton; Illa, Xavi; Traver, Estefania; Herrero, Carmen; Maldonado, Miguel J.; Villa, Rosa. 2014. "New Trends in Quantitative Assessment of the Corneal Barrier Function." Sensors 14, no. 5: 8718-8727.



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