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Sensors 2014, 14(5), 7580-7601; doi:10.3390/s140507580
Article

A DNA Microarray-Based Assay to Detect Dual Infection with Two Dengue Virus Serotypes

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Received: 17 January 2014; in revised form: 10 March 2014 / Accepted: 26 March 2014 / Published: 25 April 2014
(This article belongs to the Special Issue Microarray Sensors)
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Abstract: Here; we have described and tested a microarray based-method for the screening of dengue virus (DENV) serotypes. This DNA microarray assay is specific and sensitive and can detect dual infections with two dengue virus serotypes and single-serotype infections. Other methodologies may underestimate samples containing more than one serotype. This technology can be used to discriminate between the four DENV serotypes. Single-stranded DNA targets were covalently attached to glass slides and hybridised with specific labelled probes. DENV isolates and dengue samples were used to evaluate microarray performance. Our results demonstrate that the probes hybridized specifically to DENV serotypes; with no detection of unspecific signals. This finding provides evidence that specific probes can effectively identify single and double infections in DENV samples.
Keywords: dengue virus; humans; Aedes; microarrays dengue virus; humans; Aedes; microarrays
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Díaz-Badillo, A.; de Lourdes Muñoz, M.; Perez-Ramirez, G.; Altuzar, V.; Burgueño, J.; Mendoza-Alvarez, J.G.; Martínez-Muñoz, J.P.; Cisneros, A.; Navarrete-Espinosa, J.; Sanchez-Sinencio, F. A DNA Microarray-Based Assay to Detect Dual Infection with Two Dengue Virus Serotypes. Sensors 2014, 14, 7580-7601.

AMA Style

Díaz-Badillo A, de Lourdes Muñoz M, Perez-Ramirez G, Altuzar V, Burgueño J, Mendoza-Alvarez JG, Martínez-Muñoz JP, Cisneros A, Navarrete-Espinosa J, Sanchez-Sinencio F. A DNA Microarray-Based Assay to Detect Dual Infection with Two Dengue Virus Serotypes. Sensors. 2014; 14(5):7580-7601.

Chicago/Turabian Style

Díaz-Badillo, Alvaro; de Lourdes Muñoz, María; Perez-Ramirez, Gerardo; Altuzar, Victor; Burgueño, Juan; Mendoza-Alvarez, Julio G.; Martínez-Muñoz, Jorge P.; Cisneros, Alejandro; Navarrete-Espinosa, Joel; Sanchez-Sinencio, Feliciano. 2014. "A DNA Microarray-Based Assay to Detect Dual Infection with Two Dengue Virus Serotypes." Sensors 14, no. 5: 7580-7601.



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