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Sensors 2014, 14(5), 7580-7601; doi:10.3390/s140507580

A DNA Microarray-Based Assay to Detect Dual Infection with Two Dengue Virus Serotypes

1,* , 1
1 Department of Genetics and Molecular Biology, Centro de Investigación y de Estudios Avanzados del IPN (CINVESTAV-IPN), Mexico D.F., 07360, Mexico 2 Coordinación Académica, Universidad Autónoma de la Ciudad de México, México D.F., 09620, Mexico 3 Centro de Investigación en Micro y Nanotecnología (MICRONA), Universidad Veracruzana, Boca del Rio, Veracruz, 94294, Mexico 4 Centro Internacional de Mejoramiento de Maíz y Trigo (CIMMYT), Texcoco, Estado de Mexico, 56237, Mexico 5 Department of Physics, Centro de Investigación y de Estudios Avanzados del IPN (CINVESTAV-IPN), Mexico D.F., 07360, Mexico 6 Laboratorio Estatal de Salud Pública de Oaxaca, Servicios de Salud de Oaxaca, Oaxaca, 71257, Mexico 7 Escuela de Medicina Veterinaria y Zootecnia, Universidad Autónoma Benito Juarez de Oaxaca, 68120, Oaxaca, Mexico 8 División de Epidemiologia, Coordinación de Programas Integrados de Salud, Instituto Mexicano del Seguro Social, Mexico D.F., 06700, Mexico
* Author to whom correspondence should be addressed.
Received: 17 January 2014 / Revised: 10 March 2014 / Accepted: 26 March 2014 / Published: 25 April 2014
(This article belongs to the Special Issue Microarray Sensors)
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Here; we have described and tested a microarray based-method for the screening of dengue virus (DENV) serotypes. This DNA microarray assay is specific and sensitive and can detect dual infections with two dengue virus serotypes and single-serotype infections. Other methodologies may underestimate samples containing more than one serotype. This technology can be used to discriminate between the four DENV serotypes. Single-stranded DNA targets were covalently attached to glass slides and hybridised with specific labelled probes. DENV isolates and dengue samples were used to evaluate microarray performance. Our results demonstrate that the probes hybridized specifically to DENV serotypes; with no detection of unspecific signals. This finding provides evidence that specific probes can effectively identify single and double infections in DENV samples.
Keywords: dengue virus; humans; Aedes; microarrays dengue virus; humans; Aedes; microarrays
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Díaz-Badillo, A.; de Lourdes Muñoz, M.; Perez-Ramirez, G.; Altuzar, V.; Burgueño, J.; Mendoza-Alvarez, J.G.; Martínez-Muñoz, J.P.; Cisneros, A.; Navarrete-Espinosa, J.; Sanchez-Sinencio, F. A DNA Microarray-Based Assay to Detect Dual Infection with Two Dengue Virus Serotypes. Sensors 2014, 14, 7580-7601.

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