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Sensors 2014, 14(11), 20519-20532; doi:10.3390/s141120519

Fabrication and Evaluation of a Micro(Bio)Sensor Array Chip for Multiple Parallel Measurements of Important Cell Biomarkers

1
Centre for Research in Biosciences, Faculty of Health and Life Sciences, University of the West of England, Bristol, BS16 1QY, UK
2
QinetiQ Ltd., Malvern Technology Centre, Malvern, WR14 3PS, UK
3
Applied Enzyme Technology Ltd., Monmouth House, Mamhilad Park, Pontypool NP4 OHZ, UK
4
Uniscan Instruments Ltd., Sigma House, Burlow Rd., Buxton, Derbyshire SK17 9JB, UK
5
Gwent Electronic Materials Ltd., Monmouth House, Mamhilad Park, Pontypool NP4 OHZ, UK
6
AstraZeneca R&D, Alderley Park, Macclesfield, SK10 4TF, UK
7
Institute of Biosensing Technology, University of the West of England, Bristol, BS16 1QY, UK
Current address: Institute of Biosensing Technology, University of the West of England, Bristol, BS16 1QY, UK
Current address: Centre for Research in Translational Biomedicine, School of Biomedical and Biological Sciences, University of Plymouth, Plymouth PL4 8AA, UK
*
Authors to whom correspondence should be addressed.
Received: 21 July 2014 / Revised: 20 September 2014 / Accepted: 21 October 2014 / Published: 30 October 2014
(This article belongs to the Special Issue Printed Sensors)
View Full-Text   |   Download PDF [2122 KB, uploaded 30 October 2014]   |  

Abstract

This report describes the design and development of an integrated electrochemical cell culture monitoring system, based on enzyme-biosensors and chemical sensors, for monitoring indicators of mammalian cell metabolic status. MEMS technology was used to fabricate a microwell-format silicon platform including a thermometer, onto which chemical sensors (pH, O2) and screen-printed biosensors (glucose, lactate), were grafted/deposited. Microwells were formed over the fabricated sensors to give 5-well sensor strips which were interfaced with a multipotentiostat via a bespoke connector box interface. The operation of each sensor/biosensor type was examined individually, and examples of operating devices in five microwells in parallel, in either potentiometric (pH sensing) or amperometric (glucose biosensing) mode are shown. The performance characteristics of the sensors/biosensors indicate that the system could readily be applied to cell culture/toxicity studies. View Full-Text
Keywords: microbiosensor; screen-printing; MEMS; 96-well plate; metabolism; amperometry; cell monitoring; toxicity testing microbiosensor; screen-printing; MEMS; 96-well plate; metabolism; amperometry; cell monitoring; toxicity testing
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Pemberton, R.M.; Cox, T.; Tuffin, R.; Drago, G.A.; Griffiths, J.; Pittson, R.; Johnson, G.; Xu, J.; Sage, I.C.; Davies, R.; Jackson, S.K.; Kenna, G.; Luxton, R.; Hart, J.P. Fabrication and Evaluation of a Micro(Bio)Sensor Array Chip for Multiple Parallel Measurements of Important Cell Biomarkers. Sensors 2014, 14, 20519-20532.

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