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Sensors 2013, 13(9), 11498-11506; doi:10.3390/s130911498
Article

An Acetylcholinesterase-Based Chronoamperometric Biosensor for Fast and Reliable Assay of Nerve Agents

1,2,* , 3,4
 and 3,4
Received: 16 July 2013; in revised form: 19 August 2013 / Accepted: 27 August 2013 / Published: 30 August 2013
(This article belongs to the Special Issue Biomedical Sensors and Systems)
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Abstract: The enzyme acetylcholinesterase (AChE) is an important part of cholinergic nervous system, where it stops neurotransmission by hydrolysis of the neurotransmitter acetylcholine. It is sensitive to inhibition by organophosphate and carbamate insecticides, some Alzheimer disease drugs, secondary metabolites such as aflatoxins and nerve agents used in chemical warfare. When immobilized on a sensor (physico-chemical transducer), it can be used for assay of these inhibitors. In the experiments described herein, an AChE- based electrochemical biosensor using screen printed electrode systems was prepared. The biosensor was used for assay of nerve agents such as sarin, soman, tabun and VX. The limits of detection achieved in a measuring protocol lasting ten minutes were 7.41 × 10−12 mol/L for sarin, 6.31 × 10−12 mol /L for soman, 6.17 × 10−11 mol/L for tabun, and 2.19 × 10−11 mol/L for VX, respectively. The assay was reliable, with minor interferences caused by the organic solvents ethanol, methanol, isopropanol and acetonitrile. Isopropanol was chosen as suitable medium for processing lipophilic samples.
Keywords: biosensor; acetylcholinesterase; sarin; tabun; soman; VX; inhibitor; screen printed electrode; voltammetry; amperometry biosensor; acetylcholinesterase; sarin; tabun; soman; VX; inhibitor; screen printed electrode; voltammetry; amperometry
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Pohanka, M.; Adam, V.; Kizek, R. An Acetylcholinesterase-Based Chronoamperometric Biosensor for Fast and Reliable Assay of Nerve Agents. Sensors 2013, 13, 11498-11506.

AMA Style

Pohanka M, Adam V, Kizek R. An Acetylcholinesterase-Based Chronoamperometric Biosensor for Fast and Reliable Assay of Nerve Agents. Sensors. 2013; 13(9):11498-11506.

Chicago/Turabian Style

Pohanka, Miroslav; Adam, Vojtech; Kizek, Rene. 2013. "An Acetylcholinesterase-Based Chronoamperometric Biosensor for Fast and Reliable Assay of Nerve Agents." Sensors 13, no. 9: 11498-11506.


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