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Sensors 2013, 13(4), 4571-4580; doi:10.3390/s130404571
Article

Development of a Sensor System for the Determination of Sanitary Quality of Grapes

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Received: 1 March 2013; in revised form: 1 April 2013 / Accepted: 1 April 2013 / Published: 8 April 2013
(This article belongs to the Special Issue Novel Tools in Electrochemical Sensing)
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Abstract: n instrument for the automatic quantification of glycerol in grapes has been developed. We verify here that this analyte can be used as a benchmark of a serious disease affecting the grapevines, namely Botrytis cinerea. The core of the instrument is an amperometric biosensor consisting of a disposable screen printed electrode, generating the analytical signal thanks to a bi-enzymatic process involving glycerol dehydrogenase and diaphorase. The full automation of the analysis is realised by three micropumps and a microprocessor under control of a personal computer. The pumps allow the correct and constant dilution of the grape juice with a buffer solution also containing [Fe(CN)6]3− redox mediator and the injection of NAD+ cofactor when the baseline signal reaches a steady state; the instrument leads to automated reading of the analytical signal and the consequent data treatment. Although the analytical method is based on an amperometric technique that, owing to heavy matrix effects, usually requires an internal calibration, the analyses indicate that a unique external calibration is suitable for giving accurate responses for any grapes, both white and black ones.
Keywords: amperometric sensor; glycerol; glycerol dehydrogenase; diaphorase; Botrytis cinerea; screen printed electrodes; micropumps; microprocessor; grapes amperometric sensor; glycerol; glycerol dehydrogenase; diaphorase; Botrytis cinerea; screen printed electrodes; micropumps; microprocessor; grapes
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Zanardi, C.; Ferrari, L.; Zanfrognini, B.; Pigani, L.; Terzi, F.; Cattini, S.; Rovati, L.; Seeber, R. Development of a Sensor System for the Determination of Sanitary Quality of Grapes. Sensors 2013, 13, 4571-4580.

AMA Style

Zanardi C, Ferrari L, Zanfrognini B, Pigani L, Terzi F, Cattini S, Rovati L, Seeber R. Development of a Sensor System for the Determination of Sanitary Quality of Grapes. Sensors. 2013; 13(4):4571-4580.

Chicago/Turabian Style

Zanardi, Chiara; Ferrari, Luca; Zanfrognini, Barbara; Pigani, Laura; Terzi, Fabio; Cattini, Stefano; Rovati, Luigi; Seeber, Renato. 2013. "Development of a Sensor System for the Determination of Sanitary Quality of Grapes." Sensors 13, no. 4: 4571-4580.



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