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An Optofluidic Temperature Probe
Department of Chemical and Biological Engineering, Chalmers University of Technology, Kemivägen 10, Göteborg SE-412 96, Sweden
* Author to whom correspondence should be addressed.
Received: 26 January 2013; in revised form: 18 March 2013 / Accepted: 22 March 2013 / Published: 28 March 2013
Abstract: We report the application of a microfluidic device for semi-contact temperature measurement in picoliter volumes of aqueous media. Our device, a freely positionable multifunctional pipette, operates by a hydrodynamic confinement principle, i.e., by creating a virtual flow cell of micrometer dimensions within a greater aqueous volume. We utilized two fluorescent rhodamines, which exhibit different fluorescent responses with temperature, and made ratiometric intensity measurements. The temperature dependence of the intensity ratio was calibrated and used in a model study of the thermal activation of TRPV1 ion channels expressed in Chinese hamster ovary cells. Our approach represents a practical and robust solution to the specific problem of measuring temperature in biological experiments in vitro, involving highly localized heat generation, for example with an IR-B laser.
Keywords: Rhodamine B; Rhodamine 6G; multifunctional pipette; microfluidic device; microthermometer; temperature sensing; semi-contact; optofluidic, TRPV1
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MDPI and ACS Style
Węgrzyn, I.; Ainla, A.; Jeffries, G.D.M.; Jesorka, A. An Optofluidic Temperature Probe. Sensors 2013, 13, 4289-4302.
Węgrzyn I, Ainla A, Jeffries GDM, Jesorka A. An Optofluidic Temperature Probe. Sensors. 2013; 13(4):4289-4302.
Węgrzyn, Ilona; Ainla, Alar; Jeffries, Gavin D.M.; Jesorka, Aldo. 2013. "An Optofluidic Temperature Probe." Sensors 13, no. 4: 4289-4302.