Sensors 2013, 13(4), 4289-4302; doi:10.3390/s130404289
Article

An Optofluidic Temperature Probe

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Received: 26 January 2013; in revised form: 18 March 2013 / Accepted: 22 March 2013 / Published: 28 March 2013
(This article belongs to the Special Issue Microfluidic Devices)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: We report the application of a microfluidic device for semi-contact temperature measurement in picoliter volumes of aqueous media. Our device, a freely positionable multifunctional pipette, operates by a hydrodynamic confinement principle, i.e., by creating a virtual flow cell of micrometer dimensions within a greater aqueous volume. We utilized two fluorescent rhodamines, which exhibit different fluorescent responses with temperature, and made ratiometric intensity measurements. The temperature dependence of the intensity ratio was calibrated and used in a model study of the thermal activation of TRPV1 ion channels expressed in Chinese hamster ovary cells. Our approach represents a practical and robust solution to the specific problem of measuring temperature in biological experiments in vitro, involving highly localized heat generation, for example with an IR-B laser.
Keywords: Rhodamine B; Rhodamine 6G; multifunctional pipette; microfluidic device; microthermometer; temperature sensing; semi-contact; optofluidic, TRPV1
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MDPI and ACS Style

Węgrzyn, I.; Ainla, A.; Jeffries, G.D.M.; Jesorka, A. An Optofluidic Temperature Probe. Sensors 2013, 13, 4289-4302.

AMA Style

Węgrzyn I, Ainla A, Jeffries GDM, Jesorka A. An Optofluidic Temperature Probe. Sensors. 2013; 13(4):4289-4302.

Chicago/Turabian Style

Węgrzyn, Ilona; Ainla, Alar; Jeffries, Gavin D.M.; Jesorka, Aldo. 2013. "An Optofluidic Temperature Probe." Sensors 13, no. 4: 4289-4302.


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