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A Fast Response Highly Selective Probe for the Detection of Glutathione in Human Blood Plasma
Department of Chemistry, Portland State University, Portland, OR 97201, USA
Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, Institute of Analytical Sciences, College of Chemistry & Materials Science, Northwest University, Xi’an 710069, China
* Author to whom correspondence should be addressed.
Received: 11 April 2012; in revised form: 1 May 2012 / Accepted: 3 May 2012 / Published: 8 May 2012
Abstract: A fluorescent probe for glutathione (GSH) detection was developed. Our study indicates a possible mechanism which couples a conjugate addition and micelle-catalyzed large membered ring formation/elimination sequence. This method enables excellent selectivity towards GSH over other biological thiols such as cysteine (Cys) and homocysteine (Hcy). The proposed method is precise with a relative standard deviation (R.S.D) lower than 6% (n = 3) and has been successfully applied to determine GSH in human plasma with recoveries between 99.2% and 102.3%.
Keywords: fluorescent probe; glutathione; micelle-catalyzed; selectivity
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Guo, Y.; Yang, X.; Hakuna, L.; Barve, A.; Escobedo, J.O.; Lowry, M.; Strongin, R.M. A Fast Response Highly Selective Probe for the Detection of Glutathione in Human Blood Plasma. Sensors 2012, 12, 5940-5950.
Guo Y, Yang X, Hakuna L, Barve A, Escobedo JO, Lowry M, Strongin RM. A Fast Response Highly Selective Probe for the Detection of Glutathione in Human Blood Plasma. Sensors. 2012; 12(5):5940-5950.
Guo, Yixing; Yang, Xiaofeng; Hakuna, Lovemore; Barve, Aabha; Escobedo, Jorge O.; Lowry, Mark; Strongin, Robert M. 2012. "A Fast Response Highly Selective Probe for the Detection of Glutathione in Human Blood Plasma." Sensors 12, no. 5: 5940-5950.