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Sensors 2012, 12(5), 5940-5950; doi:10.3390/s120505940

A Fast Response Highly Selective Probe for the Detection of Glutathione in Human Blood Plasma

1 Department of Chemistry, Portland State University, Portland, OR 97201, USA 2 Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, Institute of Analytical Sciences, College of Chemistry & Materials Science, Northwest University, Xi’an 710069, China
* Author to whom correspondence should be addressed.
Received: 11 April 2012 / Revised: 1 May 2012 / Accepted: 3 May 2012 / Published: 8 May 2012
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A fluorescent probe for glutathione (GSH) detection was developed. Our study indicates a possible mechanism which couples a conjugate addition and micelle-catalyzed large membered ring formation/elimination sequence. This method enables excellent selectivity towards GSH over other biological thiols such as cysteine (Cys) and homocysteine (Hcy). The proposed method is precise with a relative standard deviation (R.S.D) lower than 6% (n = 3) and has been successfully applied to determine GSH in human plasma with recoveries between 99.2% and 102.3%.
Keywords: fluorescent probe; glutathione; micelle-catalyzed; selectivity fluorescent probe; glutathione; micelle-catalyzed; selectivity
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Guo, Y.; Yang, X.; Hakuna, L.; Barve, A.; Escobedo, J.O.; Lowry, M.; Strongin, R.M. A Fast Response Highly Selective Probe for the Detection of Glutathione in Human Blood Plasma. Sensors 2012, 12, 5940-5950.

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