Sensors 2012, 12(5), 5940-5950; doi:10.3390/s120505940
Communication

A Fast Response Highly Selective Probe for the Detection of Glutathione in Human Blood Plasma

Yixing Guo 1 email, Xiaofeng Yang 2 email, Lovemore Hakuna 1 email, Aabha Barve 1 email, Jorge O. Escobedo 1 email, Mark Lowry 1 email and Robert M. Strongin 1,* email
1 Department of Chemistry, Portland State University, Portland, OR 97201, USA 2 Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, Institute of Analytical Sciences, College of Chemistry & Materials Science, Northwest University, Xi’an 710069, China
* Author to whom correspondence should be addressed.
Received: 11 April 2012; in revised form: 1 May 2012 / Accepted: 3 May 2012 / Published: 8 May 2012
(This article belongs to the Special Issue Synthetic Fluorescent Indicators for Low Molecular Weight Organic Metabolites)
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Abstract: A fluorescent probe for glutathione (GSH) detection was developed. Our study indicates a possible mechanism which couples a conjugate addition and micelle-catalyzed large membered ring formation/elimination sequence. This method enables excellent selectivity towards GSH over other biological thiols such as cysteine (Cys) and homocysteine (Hcy). The proposed method is precise with a relative standard deviation (R.S.D) lower than 6% (n = 3) and has been successfully applied to determine GSH in human plasma with recoveries between 99.2% and 102.3%.
Keywords: fluorescent probe; glutathione; micelle-catalyzed; selectivity

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Cite This Article

MDPI and ACS Style

Guo, Y.; Yang, X.; Hakuna, L.; Barve, A.; Escobedo, J.O.; Lowry, M.; Strongin, R.M. A Fast Response Highly Selective Probe for the Detection of Glutathione in Human Blood Plasma. Sensors 2012, 12, 5940-5950.

AMA Style

Guo Y, Yang X, Hakuna L, Barve A, Escobedo JO, Lowry M, Strongin RM. A Fast Response Highly Selective Probe for the Detection of Glutathione in Human Blood Plasma. Sensors. 2012; 12(5):5940-5950.

Chicago/Turabian Style

Guo, Yixing; Yang, Xiaofeng; Hakuna, Lovemore; Barve, Aabha; Escobedo, Jorge O.; Lowry, Mark; Strongin, Robert M. 2012. "A Fast Response Highly Selective Probe for the Detection of Glutathione in Human Blood Plasma." Sensors 12, no. 5: 5940-5950.

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