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Sensors 2012, 12(12), 16710-16731; doi:10.3390/s121216710
Article

Immunoanalysis Methods for the Detection of Dioxins and Related Chemicals

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Received: 19 September 2012; in revised form: 24 October 2012 / Accepted: 2 November 2012 / Published: 5 December 2012
(This article belongs to the Special Issue Bioassays)
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Abstract: With the development of biotechnology, approaches based on antibodies, such as enzyme-linked immunosorbent assay (ELISA), active aryl hydrocarbon immunoassay (Ah-I) and other multi-analyte immunoassays, have been utilized as alternatives to the conventional techniques based on gas chromatography and mass spectroscopy for the analysis of dioxin and dioxin-like compounds in environmental and biological samples. These screening methods have been verified as rapid, simple and cost-effective. This paper provides an overview on the development and application of antibody-based approaches, such as ELISA, Ah-I, and multi-analyte immunoassays, covering the sample extraction and cleanup, antigen design, antibody preparation and immunoanalysis. However, in order to meet the requirements for on-site fast detection and relative quantification of dioxins in the environment, further optimization is needed to make these immuno-analytical methods more sensitive and easy to use.
Keywords: ELISA; dioxins; Ah-I; multi-analyte immunoassay ELISA; dioxins; Ah-I; multi-analyte immunoassay
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Tian, W.; Xie, H.Q.; Fu, H.; Pei, X.; Zhao, B. Immunoanalysis Methods for the Detection of Dioxins and Related Chemicals. Sensors 2012, 12, 16710-16731.

AMA Style

Tian W, Xie HQ, Fu H, Pei X, Zhao B. Immunoanalysis Methods for the Detection of Dioxins and Related Chemicals. Sensors. 2012; 12(12):16710-16731.

Chicago/Turabian Style

Tian, Wenjing; Xie, Heidi Q.; Fu, Hualing; Pei, Xinhui; Zhao, Bin. 2012. "Immunoanalysis Methods for the Detection of Dioxins and Related Chemicals." Sensors 12, no. 12: 16710-16731.


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