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Immunoanalysis Methods for the Detection of Dioxins and Related Chemicals
State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China
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Received: 19 September 2012; in revised form: 24 October 2012 / Accepted: 2 November 2012 / Published: 5 December 2012
(This article belongs to the Special Issue Bioassays
Abstract: With the development of biotechnology, approaches based on antibodies, such as enzyme-linked immunosorbent assay (ELISA), active aryl hydrocarbon immunoassay (Ah-I) and other multi-analyte immunoassays, have been utilized as alternatives to the conventional techniques based on gas chromatography and mass spectroscopy for the analysis of dioxin and dioxin-like compounds in environmental and biological samples. These screening methods have been verified as rapid, simple and cost-effective. This paper provides an overview on the development and application of antibody-based approaches, such as ELISA, Ah-I, and multi-analyte immunoassays, covering the sample extraction and cleanup, antigen design, antibody preparation and immunoanalysis. However, in order to meet the requirements for on-site fast detection and relative quantification of dioxins in the environment, further optimization is needed to make these immuno-analytical methods more sensitive and easy to use.
Keywords: ELISA; dioxins; Ah-I; multi-analyte immunoassay
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MDPI and ACS Style
Tian, W.; Xie, H.Q.; Fu, H.; Pei, X.; Zhao, B. Immunoanalysis Methods for the Detection of Dioxins and Related Chemicals. Sensors 2012, 12, 16710-16731.
Tian W, Xie HQ, Fu H, Pei X, Zhao B. Immunoanalysis Methods for the Detection of Dioxins and Related Chemicals. Sensors. 2012; 12(12):16710-16731.
Tian, Wenjing; Xie, Heidi Q.; Fu, Hualing; Pei, Xinhui; Zhao, Bin. 2012. "Immunoanalysis Methods for the Detection of Dioxins and Related Chemicals." Sensors 12, no. 12: 16710-16731.