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Sensors 2012, 12(12), 16037-16045; doi:10.3390/s121216037
Review

Identification of Cell-Surface Molecular Interactions under Living Conditions by Using the Enzyme-Mediated Activation of Radical Sources (EMARS) Method

1,2,3,*  and 3,4
Received: 8 October 2012; in revised form: 12 November 2012 / Accepted: 12 November 2012 / Published: 22 November 2012
(This article belongs to the Special Issue Live Cell-Based Sensors)
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Abstract: Important biological events associated with plasma membranes, such as signal transduction, cell adhesion, and protein trafficking, are mediated through the membrane microdomains. We have developed a novel method termed enzyme-mediated activation of radical sources (EMARS) to identify coclustering molecules on the cell surface under living conditions, which features a radical formation from an aryl azide reagent by horseradish peroxidase (HRP). For identification of molecules labeled by the EMARS reaction, antibody array system and mass spectrometry-based proteomics approaches are available. Spatio- temporally-regulated interaction between b1 integrin and ErbB4 involved in fibronectin-dependent cell migration and therapeutic antibody-stimulated interaction between FGFR3 and CD20 were discovered using the EMARS method.
Keywords: molecular interaction; membrane microdomains; lipid rafts; EMARS; HRP; receptor tyrosine kinases; antibody array; proteomics molecular interaction; membrane microdomains; lipid rafts; EMARS; HRP; receptor tyrosine kinases; antibody array; proteomics
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Honke, K.; Kotani, N. Identification of Cell-Surface Molecular Interactions under Living Conditions by Using the Enzyme-Mediated Activation of Radical Sources (EMARS) Method. Sensors 2012, 12, 16037-16045.

AMA Style

Honke K, Kotani N. Identification of Cell-Surface Molecular Interactions under Living Conditions by Using the Enzyme-Mediated Activation of Radical Sources (EMARS) Method. Sensors. 2012; 12(12):16037-16045.

Chicago/Turabian Style

Honke, Koichi; Kotani, Norihiro. 2012. "Identification of Cell-Surface Molecular Interactions under Living Conditions by Using the Enzyme-Mediated Activation of Radical Sources (EMARS) Method." Sensors 12, no. 12: 16037-16045.


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