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Sensors 2010, 10(7), 6347-6360; doi:10.3390/s100706347
Article

Effects of Electric Potential Treatment of a Chromium Hexacyanoferrate Modified Biosensor Based on PQQ-Dependent Glucose Dehydrogenase

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Received: 15 March 2010 / Revised: 13 May 2010 / Accepted: 23 June 2010 / Published: 28 June 2010
(This article belongs to the Special Issue Glucose Sensors)
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Abstract

A novel potential treatment technique applied to a glucose biosensor that is based on pyrroloquinoline quinone (PQQ)-dependent glucose dehydrogenase (GDH) and chromium hexacyanoferrate (CrHCF) incorporated into a platinum (Pt) electrode was demonstrated. CrHCF, serving as a mediator, was electrochemically deposited on the Pt electrode as ascertained by CV, SEM, FTIR and XPS measurements. The potential treatment of CrHCF, which converts Fe(II) to Fe(III), enables the glucose detection. The amperometric measurement linearity of the biosensor was up to 20 mM (R = 0.9923), and the detection sensitivity was 199.94 nA/mM per cm2. More importantly, this biosensor remained stable for >270 days.
Keywords: biosensor; chromium hexacyanoferrate; PQQ-dependent glucose dehydrogenase; mediator; potential treatment biosensor; chromium hexacyanoferrate; PQQ-dependent glucose dehydrogenase; mediator; potential treatment
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Tseng, T.-F.; Yang, Y.-L.; Lin, Y.-J.; Lou, S.-L. Effects of Electric Potential Treatment of a Chromium Hexacyanoferrate Modified Biosensor Based on PQQ-Dependent Glucose Dehydrogenase. Sensors 2010, 10, 6347-6360.

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